Anticorps Polyclonal de lapin anti-SKP1

SKP1 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

8

Avis (1)

N° de cat : 10990-2-AP

Synonymes

EMC19, NET19, OCP 2, OCP II, OCP2, Organ of Corti protein 2, Organ of Corti protein II, p19A, p19skp1, SIII, SKP1, SKP1A, TCEB1L

---

Galerie de données de validation

• 

  WB analysis using 10990-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10990-2-AP (SKP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10990-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10990-2-AP (SKP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10990-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10990-2-AP (SKP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 10990-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10990-2-AP (SKP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 10990-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10990-2-AP (SKP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10990-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10990-2-AP (SKP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10990-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10990-2-AP (SKP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10990-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SKP1 antibody (10990-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MCF-7, tissu cérébral de souris, tissu cérébral humain, tissu testiculaire de souris
• Résultats positifs en IHC: tissu de gliome humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

10990-2-AP cible SKP1 dans les applications de WB, IHC, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: bovin, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SKP1 Protéine recombinante Ag1457
• Nom complet: S-phase kinase-associated protein 1
• Masse moléculaire calculée: 19 kDa
• Poids moléculaire observé: 19 kDa
• Numéro d’acquisition GenBank: BC009839
• Symbole du gène: SKP1
• Identification du gène (NCBI): 6500
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SKP1(S-phase kinase-associated protein 1) is also named as EMC19, OCP2, SKP1A, TCEB1L,belongs to the SKP1 family.It is an essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription.

Publications

Species	Application	Title
human	WB	Elife; Elevated FBXO45 promotes liver tumorigenesis through enhancing IGF2BP1 ubiquitination and subsequent PLK1 upregulation.; Authors - Xiao-Tong Lin
human	WB,IHC	Pharmacol Res; Brusatol has therapeutic efficacy in non-small cell lung cancer by targeting Skp1 to inhibit cancer growth and metastasis.; Authors - Shangping Xing
human	WB	Mol Nutr Food Res; Ethanol Extract of Citrus grandis 'Tomentosa' Exerts Anticancer Effects by Targeting Skp2/p27 Pathway in Non-Small Cell Lung Cancer; Authors - Da Huang
human	WB	J Cell Physiol; The Ubiquitin Ligase SCF(FBXW7α) Promotes ATA3 Degradation.; Authors - Nan Song
human	IHC, WB	J Exp Clin Cancer Res; Long non-coding RNA CCDC183-AS1 acts AS a miR-589-5p sponge to promote the progression of hepatocellular carcinoma through regulating SKP1 expression.; Authors - He Zhu
human	WB	bioRxiv; Recruitment of FBXO22 for Targeted Degradation of NSD2; Authors - David Y Nie

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

WEI (Verified Customer) (05-11-2022)

Weak band but at correct size.

• Applications: Western Blot
• Cell Tissue Type: Mouse Heart