Anticorps Polyclonal de lapin anti-SKIV2L2

SKIV2L2 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (1)

N° de cat : 12719-2-AP

Synonymes

ATP dependent helicase SKIV2L2, Dob1, fSAP118, KIAA0052, Mtr4, SKIV2L2

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Galerie de données de validation

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  WB analysis of HepG2 using 12719-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12719-2-AP (SKIV2L2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12719-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12719-2-AP (SKIV2L2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12719-2-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12719-2-AP (SKIV2L2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12719-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12719-2-AP (SKIV2L2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12719-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12719-2-AP (SKIV2L2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 12719-2-AP

  IP result of anti-SKIV2L2 (IP:12719-2-AP, 4ug; Detection:12719-2-AP 1:300) with mouse testis tissue lysate 4000ug.

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Applications testées

• Résultats positifs en WB: cellules HepG2, tissu cérébral humain, tissu testiculaire de souris
• Résultats positifs en IP: tissu testiculaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Informations sur le produit

12719-2-AP cible SKIV2L2 dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SKIV2L2 Protéine recombinante Ag3414
• Nom complet: superkiller viralicidic activity 2-like 2 (S. cerevisiae)
• Masse moléculaire calculée: 1042 aa, 118 kDa
• Poids moléculaire observé: 118 kDa
• Numéro d’acquisition GenBank: BC028604
• Symbole du gène: SKIV2L2
• Identification du gène (NCBI): 23517
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IP	Nat Commun; TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV.; Authors - Roy Matkovic
human	IF	RNA; Human Nuclear RNAi-Defective 2 (NRDE2) is an essential RNA splicing factor.; Authors - Alan L Jiao
human	WB,IP	Nature; Nucleolar URB1 ensures 3' ETS rRNA removal to prevent exosome surveillance; Authors - Lin Shan
human	WB	Cell Genom; NRDE2 deficiency impairs homologous recombination repair and sensitizes hepatocellular carcinoma to PARP inhibitors; Authors - Yahui Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Roy (Verified Customer) (06-12-2024)

Great antibody to detect MTR4/SKIV2L2 from HeLa/Jurkat cell extracts by WB (1/1000 - 1h incubation at RT) and did work well to immunoprecipitate MTR4/SKIV2L2 (4µg/IP).

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa/Jurkat