Anticorps Polyclonal de lapin anti-SIRT2

SIRT2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 15345-1-AP

Synonymes

SIR2, SIR2 like protein 2, SIR2L, SIR2L2, SIRT2

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Galerie de données de validation

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  WB analysis of human brain using 15345-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15345-1-AP (SIRT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 15345-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 15345-1-AP (SIRT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 15345-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 15345-1-AP (SIRT2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15345-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15345-1-AP (SIRT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15345-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15345-1-AP (SIRT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 15345-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 15345-1-AP (SIRT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 15345-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15345-1-AP (SIRT2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 15345-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15345-1-AP (SIRT2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 15345-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15345-1-AP (SIRT2 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, tissu cérébral de souris, tissu rénal de rat
• Résultats positifs en IHC: tissu cardiaque humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below

Informations sur le produit

15345-1-AP cible SIRT2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SIRT2 Protéine recombinante Ag7556
• Nom complet: sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae)
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 37-45 kDa
• Numéro d’acquisition GenBank: BC003547
• Symbole du gène: SIRT2
• Identification du gène (NCBI): 22933
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The silent information regulator(SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging. SIR2 contains a 323 amino acid catalytic core domain with a NAD-binding domain and a large groove which is the likely site of catalysis. SIR2 is widely expressed, highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. This antibody is a rabbit polyclonal antibody raised against the N-terminal 352 residues of human SIR2 protein.

Publications

Species	Application	Title
mouse	WB	Cell Metab; An Acetylation Switch of the NLRP3 Inflammasome Regulates Aging-Associated Chronic Inflammation and Insulin Resistance.; Authors - Ming He ; KO Validated
mouse		Glia; Downregulated expression of lactate dehydrogenase in adult oligodendrocytes and its implication for the transfer of glycolysis products to axons; Authors - Erik Späte