Anticorps Polyclonal de lapin anti-SHH

• Phare
• Validé par KD/KO

SHH Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

67

Avis (1)

N° de cat : 20697-1-AP

Synonymes

HPE3, HLP3, HHG-1, HHG1, HHG 1

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Galerie de données de validation

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  WB analysis using 20697-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20697-1-AP (SHH antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 20697-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20697-1-AP (SHH antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 20697-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 20697-1-AP (SHH Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 20697-1-AP

  IP result of anti-SHH (IP:20697-1-AP, 4ug; Detection:20697-1-AP 1:500) with mouse liver tissue lysate 6400ug.

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  IHC staining of human pancreas cancer using 20697-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20697-1-AP (SHH antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 20697-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20697-1-AP (SHH antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 20697-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20697-1-AP (SHH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 20697-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20697-1-AP (SHH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 20697-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20697-1-AP (SHH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 20697-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20697-1-AP (SHH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 20697-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 20697-1-AP (SHH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 20697-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 20697-1-AP (SHH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 20697-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20697-1-AP (SHH Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse kidney using 20697-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20697-1-AP (SHH Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 20697-1-AP

  Immunofluorescent analysis of HepG2 cells, using SHH antibody 20697-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of mouse kidney using 20697-1-AP

  Frozen tissue section of adult mouse kidney was stained for acetylated ɑ-tubulin (magenta, Cat. No CL488-66200), CD31/PECAM-1 (white), and Shh (green, Cat. No 20697-1-AP) with DAPI as a counterstain for visualizing the nucleus (blue). acetylated ɑ-tubulin stains primary cilia and was conjugated to Coralite-488 fluorescent dye and psedocolored to magenta. CD31 stains endocardial/endothelial cells and was visualized with an Alexa Fluor 647 secondary antibody and psedocolored to white. Shh stains Sonic Hedgehog and was visualized with an Alexa Fluor 555 secondary antibody and psedocolored to green.The Image and figure legends are intellectual property of @LAF_in_the_LAB.

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  FC experiment of HepG2 using 20697-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human SHH (20697-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules LNCaP, cellules PC-3, tissu hépatique de rat, tissu hépatique de souris, tissu rénal de rat
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du pancréas humain, tissu de tumeur ovarienne humain, tissu embryonnaire de souris, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, tissu rénal de souris
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 59 publications below
• IHC: See 11 publications below
• IF: See 7 publications below

Informations sur le produit

20697-1-AP cible SHH dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris, vison
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: sonic hedgehog homolog (Drosophila)
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 45-51 kDa
• Numéro d’acquisition GenBank: NM_000193
• Symbole du gène: SHH
• Identification du gène (NCBI): 6469
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sonic hedgehog (SHH) is a member of the Hedgehog (Hh) family of proteins that control cell growth, survival, 

and fate.

What is the molecular weight of SHH?

The molecular weight of SHH is approximately 45 kDa, but it is cleaved autocatalytically to generate a 20 kDa 

N-terminal fragment and a 25 kDa C-terminal fragment responsible for hedgehog biological activity and the 

auto-processing machinery respectively (PMID: 10753901).

What is the tissue specificity of SHH?

SHH is the most broadly expressed mammalian Hh signaling molecule. During early vertebrate development, 

SHH is expressed in midline tissues such as the node, notochord, and floor plate to control the left-right and 

dorso-ventral patterning of the embryo (PMID: 12729563). SHH is expressed in fetal intestine, liver, kidney, 

and lung tissue, but is not expressed in adult tissues.

What is the cellular localization of SHH?

SHH is localized to the cell membrane. The N-terminal fragment is associated with lipid rafts at the cell surface, 

whereas the C-terminal fragment diffuses from the cell.

What is the role of SHH in the Sonic hedgehog signaling pathway?

This pathway is one of the major trafficking networks that regulate key events during developmental growth 

and patterning (PMID: 19368798). Loss of SHH signaling and regulation has been implicated in birth defects, 

tissue regeneration, stem cell renewal, and cancer growth (PMID: 15549094). The regulatory action of the SHH 

pathway is linked to the secretion, uptake, and translocation of the SHH protein; a ligand precursor, which is 

implicated in the regulation of the central nervous system polarity and neural patterning (PMID: 11130178, 

11801325).

Publications

Species	Application	Title
human	WB,IHC	Nat Commun; TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.; Authors - Rongxuan Zhu
mouse	IHC	Theranostics; Single-cell Transcriptome Profiling reveals Dermal and Epithelial cell fate decisions during Embryonic Hair Follicle Development.; Authors - Wei Ge
human	WB	Cancer Lett; An SGLT2 inhibitor modulates SHH expression by activating AMPK to inhibit the migration and induce the apoptosis of cervical carcinoma cells.; Authors - Zipeng Xie ; KD Validated
human	WB,IF	Oxid Med Cell Longev; TLR4 Modulates Senescence and Paracrine Action in Placental Mesenchymal Stem Cells via Inhibiting Hedgehog Signaling Pathway in Preeclampsia.; Authors - Yanqi Zhong
mouse	WB	Phytother Res; Total flavonoid extract from Dracocephalum moldavica L. improves pulmonary fibrosis by reducing inflammation and inhibiting the hedgehog signaling pathway; Authors - Cheng-Hui He
human	WB	Int J Oncol; Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells.; Authors - Wei Jia

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Quentin (Verified Customer) (03-28-2024)

It has worked perfectly for Western-Blot but not in Immunofluorescence on cell culture.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Neural culture