Anticorps Polyclonal de lapin anti-SHCBP1

• Phare
• Validé par KD/KO

SHCBP1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 12672-1-AP

Synonymes

PAL, SHCBP1

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Galerie de données de validation

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  WB analysis of Neuro-2a using 12672-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 12672-1-AP (SHCBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12672-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12672-1-AP (SHCBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12672-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12672-1-AP (SHCBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12672-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12672-1-AP (SHCBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12672-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12672-1-AP (SHCBP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Sp2/0 using 12672-1-AP

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 12672-1-AP (SHCBP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 12672-1-AP

  IP result of anti-SHCBP1 (IP:12672-1-AP, 4ug; Detection:12672-1-AP 1:500) with Jurkat cells lysate 2400ug.

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  IHC staining of human liver cancer using 12672-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12672-1-AP (SHCBP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 12672-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12672-1-AP (SHCBP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 12672-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12672-1-AP (SHCBP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 12672-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12672-1-AP (SHCBP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 12672-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 12672-1-AP (SHCBP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 12672-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 12672-1-AP (SHCBP1 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules Jurkat, cellules Neuro-2a, cellules Sp2/0, tissu de thymus de souris
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu d'amygdalite humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 11 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Informations sur le produit

12672-1-AP cible SHCBP1 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SHCBP1 Protéine recombinante Ag3355
• Nom complet: SHC SH2-domain binding protein 1
• Masse moléculaire calculée: 672 aa, 76 kDa
• Poids moléculaire observé: 75-85 kDa
• Numéro d’acquisition GenBank: BC030699
• Symbole du gène: SHCBP1
• Identification du gène (NCBI): 79801
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SHCBP1 was initially identified as a potential gene associated with cell proliferation in fibroblasts. SHCBP1 exerts a crucial physiological function in normal tissue development. Chen et al. showed that SHCBP1 is an essential component of FGF signaling in neural progenitor cells. SHCBP1 is also upregulated during T cell proliferation and regulates CD4+ T cell effector function in vivo. Recently, SHCBP1 was shown to be closely associated with cancer development.

Publications

Species	Application	Title
mouse	WB	Nat Neurosci; Impaired neurogenesis alters brain biomechanics in a neuroprogenitor-based genetic subtype of congenital hydrocephalus.; Authors - Phan Q Duy
human	WB,IF,IP	Genomics; SHCBP1 interacting with EOGT enhances O-GlcNAcylation of NOTCH1 and promotes the development of pancreatic cancer.; Authors - Can Yang ; KD Validated
human	WB,IHC	Am J Cancer Res; SHCBP1 regulates STAT3/c-Myc signaling activation to promote tumor progression in penile cancer.; Authors - Miao Mo ; KD Validated
human	WB	J Virol; Measles virus non-structural C protein modulates viral RNA polymerase activity by interacting with a host protein SHCBP1.; Authors - Ito Minako M ; KD Validated
mouse	WB	Mol Carcinog; Knockout of Shcbp1 sensitizes immunotherapy by regulating α-SMA positive cancer-associated fibroblasts; Authors - Qianlin Gu ; KO Validated
human	IF,IHC, WB	J Exp Clin Cancer Res; SHCBP1 promotes synovial sarcoma cell metastasis via targeting TGF-β1/Smad signaling pathway and is associated with poor prognosis.; Authors - Changliang Peng ; KD Validated