Anticorps Polyclonal de lapin anti-SHC4

• Phare
• Validé par KD/KO

SHC4 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 12641-1-AP

Synonymes

hShcD, Rai like protein, RaLP, SH2 domain protein C4, SHC transforming protein 4, SHC transforming protein D, SHC4, SHCD

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Galerie de données de validation

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  WB analysis using 12641-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12641-1-AP (SHC4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12641-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12641-1-AP (SHC4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12641-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12641-1-AP (SHC4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 12641-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12641-1-AP (SHC4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 12641-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12641-1-AP (SHC4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12641-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12641-1-AP (SHC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 12641-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12641-1-AP (SHC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12641-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12641-1-AP (SHC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 12641-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12641-1-AP (SHC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 12641-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using SHC4 antibody (12641-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 12641-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SHC4 antibody (12641-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, cellules A375, cellules HepG2, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain, tissu de muscle squelettique humain
• Résultats positifs en IHC: tissu cérébral de rat, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

12641-1-AP cible SHC4 dans les applications de WB, IHC, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SHC4 Protéine recombinante Ag3336
• Nom complet: SHC (Src homology 2 domain containing) family, member 4
• Masse moléculaire calculée: 630 aa, 69 kDa
• Poids moléculaire observé: 69 kDa
• Numéro d’acquisition GenBank: BC033907
• Symbole du gène: SHC4
• Identification du gène (NCBI): 399694
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Clin Sci (Lond); Down-regulation of the insulin signaling pathway by SHC may correlate with congenital heart disease in Chinese populations.; Authors - Zhiling Luo
human	IHC,IF	PeerJ; A new 12-gene diagnostic biomarker signature of melanoma revealed by integrated microarray analysis.; Authors - Liu Wanting W
mouse,human	WB,IHC,IF,CoIP	Cancer Lett; SHC4 orchestrates β-catenin pathway-mediated metastasis in triple-negative breast cancer by promoting Src kinase autophosphorylation; Authors - Wenjing Zhong ; KD Validated