Anticorps Polyclonal de lapin anti-SH3GLB2

• Phare
• Validé par KD/KO

SH3GLB2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 15897-1-AP

Synonymes

Endophilin B2, KIAA1848, PP6569, PP9455, SH3GLB2

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Galerie de données de validation

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  WB analysis of HeLa using 15897-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15897-1-AP (SH3GLB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 15897-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15897-1-AP (SH3GLB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat lung using 15897-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 15897-1-AP (SH3GLB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 15897-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15897-1-AP (SH3GLB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 15897-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15897-1-AP (SH3GLB2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 15897-1-AP

  IP result of anti-SH3GLB2 (IP:15897-1-AP, 4ug; Detection:15897-1-AP 1:1000) with HeLa cells lysate 2600ug.

• 

  IHC staining of human placenta using 15897-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15897-1-AP (SH3GLB2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15897-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15897-1-AP (SH3GLB2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15897-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15897-1-AP (SH3GLB2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15897-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15897-1-AP (SH3GLB2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu pulmonaire de rat, tissu pulmonaire de souris, tissu testiculaire de souris, tissu testiculaire humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu cérébral de souris, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

15897-1-AP cible SH3GLB2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SH3GLB2 Protéine recombinante Ag8712
• Nom complet: SH3-domain GRB2-like endophilin B2
• Masse moléculaire calculée: 395 aa, 44 kDa
• Poids moléculaire observé: 47-50 kDa
• Numéro d’acquisition GenBank: BC014635
• Symbole du gène: SH3GLB2
• Identification du gène (NCBI): 56904
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SH3GLB2 is an endophilin-related protein family with 395-amino acid protein and contains an N-terminal domain, a central coiled-coil region, and a C-terminal SH3 domain. SH3GLB2 is expressed in many tissues, including skeletal muscle, adipocytes, brain, lung, colon, and mammary gland. SH3GLB2 was expressed in the cytoplasm of transfected HeLa cells and was excluded from nuclei(PMID: 11161816).

Publications

Species	Application	Title
human	WB	Brain Pathol; Neuronal Susceptibility to Beta-Amyloid Toxicity and Ischemic Injury Involves Histone Deacetylase-2 Regulation of Endophilin-B1.; Authors - David B Wang
mouse	WB,IF	J Biol Chem; Endophilin B2 facilitates endosome maturation in response to growth factor stimulation, autophagy induction, and influenza A virus infection.; Authors - Jacob M Serfass ; KO Validated