Anticorps Monoclonal anti-SH3BP5

SH3BP5 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, poulet, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

1B9D4

N° de cat : 67841-1-Ig

Synonymes

SAB, SH3 domain binding protein 5, SH3BP 5, SH3BP5

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Galerie de données de validation

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  WB analysis of mouse brain using 67841-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67841-1-Ig (SH3BP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of chicken brain using 67841-1-Ig

  chicken brain tissue were subjected to SDS PAGE followed by western blot with 67841-1-Ig (SH3BP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67841-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67841-1-Ig (SH3BP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit brain using 67841-1-Ig

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 67841-1-Ig (SH3BP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules HeLa, cellules Jurkat, tissu cérébral de lapin, tissu cérébral de poulet, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67841-1-Ig cible SH3BP5 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, poulet, rat, souris

• Réactivité: Humain, Lapin, porc, poulet, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SH3BP5 Protéine recombinante Ag30252
• Nom complet: SH3-domain binding protein 5 (BTK-associated)
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC010123
• Symbole du gène: SH3BP5
• Identification du gène (NCBI): 9467
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SH3BP5(SH3 domain-binding protein 5) is also named as SAB. It is previously isolated in a screen for proteins capable of binding to the Src homology 3 (SH3) domain of Bruton's tyrosine kinase (Btk) and could play a role in targeting JNK and Btk to mitochondria and/or mediating cross-talk between the JNK and Btk signalling pathways. It has 2 isoforms produced by alternative splicing with the molecular mass of 50 kDa and 32 kDa. There are two bands of 83 kDa and 70 kDa can be detected in A431 and the larger species may correspond to splice variants of SH3BP5 or SH3BP5 proteins that have undergone post-translational modification(PMID:12167088). In addition, we detect the band of 32 kDa as the isoform 2 of SH3BP5 and another band of 62 kDa besides the 47 kDa and 80 kDa.