• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-SGLT2

SGLT2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 24654-1-AP

Synonymes

Na(+)/glucose cotransporter 2, SGLT2, SLC5A2, Sodium/glucose cotransporter 2



Applications testées

Résultats positifs en WBtissu rénal de souris, cellules A549, tissu rénal de rat
Résultats positifs en IHCtissu rénal humain, tissu rénal de rat
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochimie (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

24654-1-AP cible SGLT2 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéerat, Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène SGLT2 Protéine recombinante Ag19163
Nom complet solute carrier family 5 (sodium/glucose cotransporter), member 2
Masse moléculaire calculée 672 aa, 73 kDa
Poids moléculaire observé 69-70 kDa
Numéro d’acquisition GenBankBC131542
Symbole du gène SGLT2
Identification du gène (NCBI) 6524
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sodium-glucose cotransporter 2 (SGLT2), encoded by SLC5A2, utilizes the electrochemical sodium gradient to transport glucose against the cell's internal concentration gradient. Mainly expressed on brush border membrane (BBM) of epithelial cells in the early segment of the proximal tubule, SGLT2 mediates most of the glucose reabsorption by the kidney overall. Inhibition of SGLT2 could improve glucose homeostasis of diabetic patients, which has been considered as a novel strategy for diabetes treatment.

Protocole

Product Specific Protocols
WB protocol for SGLT2 antibody 24654-1-APDownload protocol
IHC protocol for SGLT2 antibody 24654-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Circulation

Sodium Glucose Cotransporter-2 Inhibitor Empagliflozin Reduces Infarct Size Independently of Sodium Glucose Cotransporter-2

Authors - Sha Chen
humanIHC

Cancer Lett

SGLT2 promotes pancreatic cancer progression by activating the Hippo signaling pathway via the hnRNPK-YAP1 axis.

Authors - Dianyun Ren
humanWB

Mol Metab

Sodium-glucose co-transporter2 expression and inflammatory activity in diabetic atherosclerotic plaques: Effects of sodium-glucose co-transporter2 inhibitor treatment.

Authors - Nunzia D'Onofrio
  • KD Validated
mouseIHC

Int J Mol Sci

Redefining the Role of ADAM17 in Renal Proximal Tubular Cells and Its Implications in an Obese Mouse Model of Pre-Diabetes.

Authors - Vanesa Palau
mouseIF

Metabolism

Sodium-glucose cotransporter 2 inhibition attenuates protein overload in renal proximal tubule via suppression of megalin O-GlcNacylation in progressive diabetic nephropathy.

Authors - Hitomi Otomo
humanWB

Front Pharmacol

Empagliflozin Alleviates Hepatic Steatosis by Activating the AMPK-TET2-Autophagy Pathway in vivo and in vitro.

Authors - Ting Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Elina (Verified Customer) (03-04-2019)

The antibody is quite specific, used at a 1:200 dilution in mouse kidney. Comparison to a secondary only staining shows some background. Overall it appears to work well in paraffin embedded tissues, (5um thick).

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: mouse kidney
SGLT2 Antibody Immunofluorescence, validation (1:200 dilution) in mouse kidney (Cat no:24654-1-AP)