Anticorps Polyclonal de lapin anti-SGCG

SGCG Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 18102-1-AP

Synonymes

35DAG, A4, DAGA4, DMDA, DMDA1, Gamma sarcoglycan, Gamma SG, LGMD2C, MAM, SCARMD2, SCG3, SGCG, TYPE

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Galerie de données de validation

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  WB analysis using 18102-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18102-1-AP (SGCG antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 18102-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18102-1-AP (SGCG antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 18102-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 18102-1-AP (SGCG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 18102-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18102-1-AP (SGCG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 18102-1-AP

  IP result of anti-SGCG (IP:18102-1-AP, 4ug; Detection:18102-1-AP 1:1000) with mouse heart tissue lysate 4000ug.

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  IHC staining of human heart using 18102-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 18102-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 18102-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 18102-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 18102-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 18102-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 18102-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 18102-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 18102-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 18102-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 18102-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 18102-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu cardiaque de rat, tissu cardiaque humain, tissu de muscle squelettique de souris, tissu de muscle squelettique humain
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu cardiaque humain, tissu cutané humain, tissu ovarien humain, tissu placentaire humain, tissu pulmonaire humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

18102-1-AP cible SGCG dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SGCG Protéine recombinante Ag12402
• Nom complet: sarcoglycan, gamma (35kDa dystrophin-associated glycoprotein)
• Masse moléculaire calculée: 291 aa, 32 kDa
• Poids moléculaire observé: 35-38 kDa
• Numéro d’acquisition GenBank: BC074777
• Symbole du gène: SGCG
• Identification du gène (NCBI): 6445
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB,IF,IP	Skelet Muscle; Novel γ-sarcoglycan interactors in murine muscle membranes.; Authors - Tara C Smith
mouse	IHC	PLoS One; Peptide-conjugated phosphodiamidate oligomer-mediated exon skipping has benefits for cardiac function in mdx and Cmah-/-mdx mouse models of Duchenne muscular dystrophy.; Authors - Alison M Blain
human	IF	PLoS One; Correlation of Utrophin Levels with the Dystrophin Protein Complex and Muscle Fibre Regeneration in Duchenne and Becker Muscular Dystrophy Muscle Biopsies.; Authors - Narinder Janghra
human	IF	Hum Gene Ther Methods; Optimisation of internally deleted dystrophin constructs.; Authors - Mojgan Reza
mouse	IHC	Mol Vis; Identification of HMX1 target genes: a predictive promoter model approach.; Authors - Boulling Arnaud A