Anticorps Polyclonal de lapin anti-SFXN3

• Phare
• Validé par KD/KO

SFXN3 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 15156-1-AP

Synonymes

SFX3, SFXN3, sideroflexin 3

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Galerie de données de validation

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  WB analysis of HeLa using 15156-1-AP

  WB result of SFXN3 antibody (15156-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SFXN3 transfected HeLa cells.

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  WB analysis using 15156-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15156-1-AP (SFXN3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15156-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15156-1-AP (SFXN3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15156-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15156-1-AP (SFXN3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 15156-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15156-1-AP (SFXN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 15156-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15156-1-AP (SFXN3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 15156-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15156-1-AP (SFXN3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15156-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15156-1-AP (SFXN3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15156-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15156-1-AP (SFXN3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of BxPC-3 using 15156-1-AP

  Immunofluorescent analysis of BxPC-3 cells using 15156-1-AP (SFXN3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules C6, cellules HeLa, cellules Neuro-2a, cellules NIH/3T3, cellules SH-SY5Y, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules BxPC-3

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

15156-1-AP cible SFXN3 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SFXN3 Protéine recombinante Ag7203
• Nom complet: sideroflexin 3
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC000124
• Symbole du gène: SFXN3
• Identification du gène (NCBI): 81855
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SFXN3, a mitochondrial membrane protein, is a member of sideroflexin family. Serum anti-SFXN3 autoantibody was elevated in early stage of the oral squamous cell carcinoma significantly, indicating its clinical value for diagnosis of oral squamous cell carcinoma.

Publications

Species	Application	Title
human	WB	Nat Chem Biol; Metabolic determinants of cancer cell sensitivity to canonical ferroptosis inducers.; Authors - Mariluz Soula
human	IHC	PeerJ; Identification of iron metabolism-related genes as prognostic indicators for papillary thyroid carcinoma: a retrospective study; Authors - Tiefeng Jin
human,mouse	WB	Nucleic Acids Res; Precise genome editing of the Kozak sequence enables bidirectional and quantitative modulation of protein translation to anticipated levels without affecting transcription; Authors - Jingke Xie
human	WB	Am J Respir Crit Care Med; Distinct Cancer-Promoting Stromal Gene Expression Depending on Lung Function.; Authors - Brian J Sandri