Anticorps Polyclonal de lapin anti-SFRS5

SFRS5 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (1)

N° de cat : 16237-1-AP

Synonymes

Delayed early protein HRS, HRS, Pre mRNA splicing factor SRP40, SFRS5, SRP40, SRSF5

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Galerie de données de validation

• 

  WB analysis of mouse testis using 16237-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16237-1-AP (SFRS5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 16237-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 16237-1-AP (SFRS5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

16237-1-AP cible SFRS5 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SFRS5 Protéine recombinante Ag8988
• Nom complet: splicing factor, arginine/serine-rich 5
• Masse moléculaire calculée: 272 aa, 31 kDa
• Numéro d’acquisition GenBank: BC018823
• Symbole du gène: SFRS5
• Identification du gène (NCBI): 6430
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SR proteins own a characteristic C-terminal Ser/Arg-rich repeat (RS domain) of variable length and constitute a family of highly conserved nuclear phosphoproteins that can function as both essential and alternative pre-mRNA splicing factors [PMID:7556075]. The binding of SR proteins to pre-mRNA appears to be one of the earliest steps in spliceosome assembly, enhancing the binding of U1 snRNP to the 5' splice site to form a commitment complex [PMID:1577277 ]. Alternative splicing has a major role in the regulation of gene expression. SFRS5 belongs to the serine/arginine (SR) protein family of regulators of alternative pre-mRNA splicing [PMID:9244433].

Publications

Species	Application	Title
human	WB	Cell Metab; NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.; Authors - Mi Kyung Park
human	WB	JCI Insight; Splicing factor SRSF6 mediates pleural fibrosis.; Authors - Li-Mei Liang
mouse		J Dent Res; Mutations Causing X-Linked Amelogenesis Imperfecta Alter miRNA Formation from Amelogenin Exon4; Authors - R Shemirani
mouse	WB	Cell Rep; Aspartyl-tRNA synthetase 2 orchestrates iron-sulfur metabolism in hematopoietic stem cells via fine-tuning alternative RNA splicing; Authors - Xuan Gu
human	IF	Molecules; L-Arginine Enhances Oral Keratinocyte Proliferation under High-Glucose Conditions via Upregulation of CYP1A1, SKP2, and SRSF5; Authors - Junhe Shi

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Yan (Verified Customer) (04-28-2021)

Signals was observed in the nucleus when I performed immunofluorescence.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Osteosarcoma Epithelial Cells