Anticorps Polyclonal de lapin anti-SFRP4

• Phare
• Validé par KD/KO

SFRP4 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 15328-1-AP

Synonymes

FRP 4, FRPHE, sFRP 4, SFRP4

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Galerie de données de validation

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  WB analysis of HEK-293 using 15328-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15328-1-AP (SFRP4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15328-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15328-1-AP (SFRP4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15328-1-AP

  IP result of anti-SFRP4 (IP:15328-1-AP, 4ug; Detection:15328-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IHC staining of human breast cancer using 15328-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15328-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 15328-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 15328-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin cancer using 15328-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin cancer using 15328-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin cancer using 15328-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15328-1-AP (SFRP4 antibody) at dilution of 1:200 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer de la peau humain, tissu testiculaire de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 7 publications below
• IF: See 2 publications below

Informations sur le produit

15328-1-AP cible SFRP4 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SFRP4 Protéine recombinante Ag6909
• Nom complet: secreted frizzled-related protein 4
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 41-51 kDa, 55 kDa
• Numéro d’acquisition GenBank: BC058911
• Symbole du gène: SFRP4
• Identification du gène (NCBI): 6424
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRP4 functions as a suppressor of tumor growth via inhibition of the Wnt signaling pathway.Downregulation or deletion of SFRP4 has been reported in several cancers and cell lines such as endometrial cancer, ovarian cancer, lung cancer,and others. This antibody got 41-51 kDa and 55 kDa bands in western blotting maybe due to differential posttranslational protein modification.

Publications

Species	Application	Title
human	IHC	Nat Commun; Myofibroblast transcriptome indicates SFRP2hi fibroblast progenitors in systemic sclerosis skin.; Authors - Tracy Tabib
human	IHC	Sci Rep; Expression profiling of prostate cancer tissue delineates genes associated with recurrence after prostatectomy.; Authors - Martin Mørck Mortensen
mouse	WB	Cell Physiol Biochem; Differential Patterns of Secreted Frizzled-Related Protein 4 (SFRP4) in Adipocyte Differentiation: Adipose Depot Specificity.; Authors - Hua Guan ; KD Validated
human,mouse	WB,IHC	Gene Ther; Effect of nanoparticle-mediated delivery of SFRP4 siRNA for treating Dupuytren disease.; Authors - Rujue Jin ; KD Validated
human	IHC	Sci Rep; SFRP4 gene expression is increased in aggressive prostate cancer.; Authors - Elise Sandsmark
mouse	WB	Endocrinology; SFRP4 Is a Negative Regulator of Ovarian Follicle Development and Female Fertility.; Authors - Gustavo Zamberlam ; KO Validated