Anticorps Polyclonal de lapin anti-SFRP2

SFRP2 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 12189-1-AP

Synonymes

FRP 2, FRP2, FRP-2, SARP 1, SARP1, SDF 5, sFRP 2, SFRP2, UNQ361/PRO697

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Galerie de données de validation

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  WB analysis using 12189-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12189-1-AP (SFRP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 12189-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using SFRP2 antibody (12189-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de rat, tissu cardiaque de souris
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below

Informations sur le produit

12189-1-AP cible SFRP2 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SFRP2 Protéine recombinante Ag2853
• Nom complet: secreted frizzled-related protein 2
• Masse moléculaire calculée: 295 aa, 34 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC008666
• Symbole du gène: SFRP2
• Identification du gène (NCBI): 6423
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Secreted frizzled-related protein 2 (Sfrp2) is a secreted glycoprotein molecule containing an N terminal cysteine-rich domain, which is typically 30-50% identical to the putative Wnt-binding site of the frizzled receptor, and a C terminal heparin-binding domain with weak homology to netrins. It has been implicated in diverse cellular processes, including embryogenesis, regulation of cell apoptosis, and cell differentiation. Methylation of this gene is a potential marker for the presence of colorectal cancer.

Publications

Species	Application	Title
human	WB	Cell Res; Comprehensive profiling reveals mechanisms of SOX2-mediated cell fate specification in human ESCs and NPCs.; Authors - Chenlin Zhou
human	WB	Front Oncol; Fucoidan Inhibits the Progression of Hepatocellular Carcinoma via Causing lncRNA LINC00261 Overexpression.; Authors - Danhui Ma
human	WB	Biomed Pharmacother; MicroRNA-500a promotes migration and invasion in hepatocellular carcinoma by activating the Wnt/β-catenin signaling pathway.; Authors - Yuntao Guo
mouse	WB	PLoS One; Digital gene expression tag profiling analysis of the gene expression patterns regulating the early stage of mouse spermatogenesis.; Authors - Zhang Xiujun X
		Physiol Genomics; Tenotomy Immobilization as a Model to Investigate Skeletal Muscle Fibrosis (with Emphasis on Secreted Frizzled-Related Protein 2).; Authors - Uğur Akpulat
mouse	WB	Psychiatry Investig; m6A Methyltransferase METTL3 Reduces Hippocampal Neuron Apoptosis in a Mouse Model of Autism Through the MALAT1/SFRP2/Wnt/β-catenin Axis; Authors - Yue Ming