Anticorps Polyclonal de lapin anti-SFPQ

• Phare
• Validé par KD/KO

SFPQ Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugaison

Non conjugué

Publications

24

N° de cat : 15585-1-AP

Synonymes

100 kDa DNA pairing protein, hPOMp100, POMP100, PSF, PTB associated splicing factor, SFPQ

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Galerie de données de validation

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  WB analysis of HeLa using 15585-1-AP

  WB result of SFPQ antibody (15585-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SFPQ transfected HeLa cells.

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  WB analysis using 15585-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15585-1-AP (SFPQ antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 15585-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 15585-1-AP (SFPQ Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 15585-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 15585-1-AP (SFPQ Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 15585-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15585-1-AP (SFPQ Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 15585-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15585-1-AP (SFPQ Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15585-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15585-1-AP (SFPQ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 15585-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15585-1-AP (SFPQ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 15585-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15585-1-AP (SFPQ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 15585-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 15585-1-AP (SFPQ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15585-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15585-1-AP (SFPQ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15585-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15585-1-AP (SFPQ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 15585-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15585-1-AP (SFPQ antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 15585-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15585-1-AP (SFPQ antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 15585-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 15585-1-AP (SFPQ antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules BxPC-3, cellules Jurkat, cellules MCF-7, cellules PC-3, cellules Y79, tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IHC: tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 16 publications below
• IHC: See 3 publications below
• IF: See 6 publications below
• IP: See 2 publications below
• ELISA: See 1 publications below
• RIP: See 4 publications below

Informations sur le produit

15585-1-AP cible SFPQ dans les applications de WB, IHC, IF/ICC, IP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SFPQ Protéine recombinante Ag7181
• Nom complet: splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated)
• Masse moléculaire calculée: 76 kDa
• Poids moléculaire observé: 90-100 kDa
• Numéro d’acquisition GenBank: BC051192
• Symbole du gène: SFPQ
• Identification du gène (NCBI): 6421
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SFPQ, also named PSF, encodes a nuclear factor implicated in the splicing and regulation of gene expression. SFPQ probably forms a heteromer with NONO and participates in DNA pairing and DNA break repair program. Very recently SFPQ was identified as a downstream target of tau, complete nuclear depletion and cytoplasmic accumulation of SFPQ were shown in the neurons and astrocytes of brains with Alzheimer's disease (AD), more strikingly, reduced SFPQ levels may progress together with tau pathology, these observation strongly suggests the important role of SFPQ pathology in neurodegenerative diseases including AD. SFPQ encompasses 707 amino acids and has a molecular weight of 76 kDa, although it typically migrates on a sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel at an apparent molecular weight of ∼100 kDa. Proteolytic cleavage products of apparent molecular weights of 47 and 68 kDa, and an alternatively spliced form of 669 amino acids, have also been described in various cell types. (PMID: 25832716). Splicing Factor Proline and Glutamine rich (SFPQ) as the most significant intron-retaining transcript across diverse ALS-causing mutations (VCP, SOD1 and FUS). SFPQ protein binds extensively to its retained intron, which exhibits high cytoplasmic abundance in VCP mutation compared with controls. Crucially, the protein is less abundant in the nuclei of VCP mutation cultures and is ultimately lost from nuclei of MNs in mouse models (SOD1mu and VCP mutation transgenic mouse models) and human sporadic ALS post-mortem samples. In summary, our study implicates SFPQ IR and nuclear loss as general molecular hallmarks of familial and sporadic ALS.

Publications

Species	Application	Title
human	WB	Cell Res; Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.; Authors - Shaojun Zhang
human	WB	Nat Commun; K235 acetylation couples with PSPC1 to regulate the m6A demethylation activity of ALKBH5 and tumorigenesis; Authors - Xiao-Lan Zhang
human	IF	Hepatology; Splicing Regulator p54nrb /NONO Enhances Carcinogenesis Through Oncogenic Isoform Switch of BIN1 in Hepatocellular Carcinoma.; Authors - Zhixiang Hu
human	WB	Nat Commun; PMID: 31792210; Authors - Sunwang Xu
human	WB	Dev Cell; Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.; Authors - Yanni Ma
human	WB	Cell Rep; hnRNPA2B1 represses the disassembly of arsenite-induced stress granules and is essential for male fertility; Authors - Xiaoli Wang