Anticorps Polyclonal de lapin anti-SFMBT2

SFMBT2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris et plus (1)

Applications

WB, CoIP, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 25256-1-AP

Synonymes

KIAA1617, SFMBT2

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Galerie de données de validation

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  WB analysis using 25256-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25256-1-AP (SFMBT2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 25256-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25256-1-AP (SFMBT2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 25256-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 25256-1-AP (SFMBT2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules NIH/3T3, tissu cérébral de rat, tissu splénique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

25256-1-AP cible SFMBT2 dans les applications de WB, CoIP, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SFMBT2 Protéine recombinante Ag19379
• Nom complet: Scm-like with four mbt domains 2
• Masse moléculaire calculée: 894 aa, 101 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC152430
• Symbole du gène: SFMBT2
• Identification du gène (NCBI): 57713
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SFMBT2 (Scm like with four mbt domains 2), also named as KIAA1617. It is located in Nucleus and ubiquitous expression in thyroid and ovary. The calculated molecular weight of SFMBT2 is 100 kDa. Although mammalian SFMBTs have similar structural features with PcG protein dSFMBT, biological function and target genes of SFMBT have been not investigated well. Lee K and Na W investigated biological function of human SFMBT2 that interacted with YY1 using AR (androgen receptor)-negative DU145 prostate cancer cells. They found that human SFMBT2 represses HOXB13 gene expression via its association with methylated histones H3 and H4 that are transcriptional repression marks. Furthermore, their findings indicate that SFMBT2 may be involved in regulation of cell growth of DU145 prostate cancer cells (PMID: 23385818).

Publications

Species	Application	Title
mouse		Cell Stem Cell; Overcoming Intrinsic H3K27me3 Imprinting Barriers Improves Post-implantation Development after Somatic Cell Nuclear Transfer.; Authors - Le-Yun Wang
		Int J Mol Med; SFMBT2 positively regulates SOX9 and chondrocyte proliferation.; Authors - Safdar Hussain
human	WB,CoIP	Biol Direct; Acetylation- and ubiquitination-regulated SFMBT2 acts as a tumor suppressor in clear cell renal cell carcinoma; Authors - Qingpeng Xie