Anticorps Polyclonal de lapin anti-SFI1

• Phare
• Validé par KD/KO

SFI1 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 13550-1-AP

Synonymes

hSFI1, hSfi1p, KIAA0542, PISD, Protein SFI1 homolog, RP5 858B16.1, SFI1

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Galerie de données de validation

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  WB analysis of A549 using 13550-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13550-1-AP (SFI1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 13550-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13550-1-AP (SFI1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13550-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13550-1-AP (SFI1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 13550-1-AP

  Immunofluorescent analysis of HepG2 cells using 13550-1-AP (SFI1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 13550-1-AP

  Immunofluorescent analysis of Hela cells, using SFI1 antibody 13550-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HepG2, tissu pulmonaire de souris
• Résultats positifs en IF/ICC: cellules HepG2, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

13550-1-AP cible SFI1 dans les applications de WB, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SFI1 Protéine recombinante Ag4268
• Nom complet: Sfi1 homolog, spindle assembly associated (yeast)
• Masse moléculaire calculée: 1211 aa, 134 kDa
• Poids moléculaire observé: 148 kDa
• Numéro d’acquisition GenBank: BC033613
• Symbole du gène: SFI1
• Identification du gène (NCBI): 9814
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	CoIP	EBioMedicine; PARP inhibitors suppress tumours via centrosome error-induced senescence independent of DNA damage response; Authors - Wei Yue
human	WB	J Cell Biol; SFI1 promotes centriole duplication by recruiting USP9X to stabilize the microcephaly protein STIL.; Authors - Andrew Kodani
human	WB	Sci Signal; Aurora B opposes PP1 function in mitosis by phosphorylating the conserved PP1-binding RVxF motif in PP1 regulatory proteins.; Authors - Isha Nasa
mouse	WB	Sci Rep; Dietary glucoraphanin prevents the onset of psychosis in the adult offspring after maternal immune activation.; Authors - Akiko Matsuura
human	WB	J Cell Sci; Differential requirements for the EF-hands of human centrin2 in primary ciliogenesis and nucleotide excision repair.; Authors - Ebtissal M Khouj
human	IF	EMBO J; Human SFI1 and Centrin form a complex critical for centriole architecture and ciliogenesis; Authors - Marine H Laporte ; KD Validated