Anticorps Polyclonal de lapin anti-SF3B4

• Phare
• Validé par KD/KO

SF3B4 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 10482-1-AP

Synonymes

SAP 49, SAP49, SF3B4, SF3b49, SF3b50, Splicing factor 3B subunit 4

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Galerie de données de validation

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  WB analysis of HEK-293T using 10482-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 10482-1-AP (SF3B4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 10482-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 10482-1-AP (SF3B4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10482-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10482-1-AP (SF3B4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10482-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10482-1-AP (SF3B4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10482-1-AP

  IP result of anti-SF3B4 (IP:10482-1-AP, 4ug; Detection:10482-1-AP 1:500) with HeLa cells lysate 1600ug.

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  IHC staining of human pancreas cancer using 10482-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10482-1-AP (SF3B4 antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HEK-293, cellules PC-3
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

10482-1-AP cible SF3B4 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SF3B4 Protéine recombinante Ag0737
• Nom complet: splicing factor 3b, subunit 4, 49kDa
• Masse moléculaire calculée: 44 aa, 3 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC004273
• Symbole du gène: SF3B4
• Identification du gène (NCBI): 10262
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SF3B4, also named as Pre-mRNA-splicing factor SF3b 49 kDa subunit, is a 424 amino acid protein, which belongs to the SF3B4 family. SF3B4 is a subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. SF3B4 has been found in complex 'B' and 'C' as well. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron. The calculated molecular weight of SF3B4 is 44 kDa. Based on its predicted molecular mass and the observed migration of the 50kDa cross-linked species on two-dimensional gels.(PMID: 9614130)

Publications

Species	Application	Title
human	WB	Clin Transl Med; LncRNA BC promotes lung adenocarcinoma progression by modulating IMPAD1 alternative splicing; Authors - Qi Wen Chen
human	WB	Cell Chem Biol; Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer.; Authors - Li Wang
human	WB	iScience; Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.; Authors - Mio Iwasaki
human,rat	WB	J Mol Cell Cardiol; Remodeling of the m6A landscape in the heart reveals few conserved post-transcriptional events underlying cardiomyocyte hypertrophy.; Authors - Scott A Hinger
human	WB,IHC	Cell Death Dis; SF3B4 promotes ovarian cancer progression by regulating alternative splicing of RAD52.; Authors - Yuchao Diao ; KD Validated
	WB	bioRxiv; Sf3b4 mutation in Xenopus tropicalis causes RNA splicing defects followed by massive gene dysregulation that disrupt cranial neural crest development; Authors - Casey Griffin