Anticorps Polyclonal de lapin anti-SF3B3

SF3B3 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, RIP, ELISA

Conjugaison

Non conjugué

Publications

19

N° de cat : 14577-1-AP

Synonymes

SF3b130, SAP130, SAP 130, RSE1, Pre-mRNA-splicing factor SF3b 130 kDa subunit

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Galerie de données de validation

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  WB analysis using 14577-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14577-1-AP (SF3B3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 14577-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14577-1-AP (SF3B3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 14577-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 14577-1-AP (SF3B3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 14577-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14577-1-AP (SF3B3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14577-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14577-1-AP (SF3B3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of rat brain using 14577-1-AP

  IP result of anti-SF3B3 (IP:14577-1-AP, 4ug; Detection:14577-1-AP 1:5000) with rat brain tissue lysate 4800ug.

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  IHC staining of human gliomas using 14577-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14577-1-AP (SF3B3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung cancer using 14577-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14577-1-AP (SF3B3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 14577-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14577-1-AP (SF3B3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 14577-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14577-1-AP (SF3B3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 14577-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14577-1-AP (SF3B3 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of mouse heart using 14577-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using SF3B3 antibody (14577-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse heart using 14577-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using SF3B3 antibody (14577-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 14577-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using SF3B3 antibody (14577-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 14577-1-AP

  Immunofluorescent analysis of Hela cells, using SF3B3 antibody 14577-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, tissu cardiaque de rat, tissu cardiaque de souris, tissu cérébral de rat, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de rat
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu cérébral de rat, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cardiaque de souris,
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 16 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

14577-1-AP cible SF3B3 dans les applications de WB, IHC, IF/ICC, IF-P, IP, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SF3B3 Protéine recombinante Ag5980
• Nom complet: splicing factor 3b, subunit 3, 130kDa
• Masse moléculaire calculée: 136 kDa
• Poids moléculaire observé: 130-135 kDa
• Numéro d’acquisition GenBank: BC003146
• Symbole du gène: SF3B3
• Identification du gène (NCBI): 23450
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Introns are removed from nuclear pre-mRNA in 2-step transesterification reactions. Splicing occured in a large ribonucleoprotein particle, called the spliceosome. Spliceosomal intermediate complexes form on pre-mRNA in the order E, A, B, and C, with the catalytic reactions occurring in complex C. U2 small nuclear ribonucleoproteins are one of the proteins essential for spliceosome assembly and mRNA splicing. Functional U2 snRNP is composed of a 12S unit and 2 splicing factors, SF3A, which is composed of 3 proteins, and SF3B, which conposed of 4 proteins. SF3B3 is one of SF3B, and it's required for 'A' complex assembly formed by the stable binding of U2 snRNP to the brachpoint sequence(BPS) in pre-mRNA.

Publications

Species	Application	Title
human	WB	Gastroenterology; Enterotoxigenic Bacteroides fragilis promotes intestinal inflammation and malignancy by inhibiting exosomes-packaged miR-149-3p.; Authors - Yingying Cao
		Genome Res; RNA structure replaces the need for U2AF2 in splicing.; Authors - Chien-Ling Lin
human	WB	Nat Commun; Splicing modulators act at the branch point adenosine binding pocket defined by the PHF5A-SF3b complex.; Authors - Teng Teng
human	RIP	Nucleic Acids Res; Corrigendum to article "Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation''.; Authors - Ruibing Chen
human	WB	Mol Neurodegener; Nuclear speckle specific hnRNP D-like prevents age- and AD-related cognitive decline by modulating RNA splicing.; Authors - Qingyang Zhang
human	WB	Nucleic Acids Res; Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation.; Authors - Ruibing Chen