Anticorps Polyclonal de lapin anti-SF3B2

SF3B2 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 10919-1-AP

Synonymes

SAP 145, SAP145, SF3B145, SF3b150, SF3B2, Splicing factor 3B subunit 2

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Galerie de données de validation

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  WB analysis using 10919-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10919-1-AP (SF3B2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 10919-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10919-1-AP (SF3B2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10919-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10919-1-AP (SF3B2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 10919-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10919-1-AP (SF3B2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 10919-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10919-1-AP (SF3B2 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HEK-293 using 10919-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SF3B2 antibody (10919-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules K-562
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below

Informations sur le produit

10919-1-AP cible SF3B2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SF3B2 Protéine recombinante Ag1009
• Nom complet: splicing factor 3b, subunit 2, 145kDa
• Masse moléculaire calculée: 100 kDa
• Poids moléculaire observé: 130-140 kDa
• Numéro d’acquisition GenBank: BC007610
• Symbole du gène: SF3B2
• Identification du gène (NCBI): 10992
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SF3B (splicing factor 3B) is a U2 snRNP-associated protein complex essential for spliceosome assembly. SF3B complex contains the spliceosome-associated protein (SAP) 49, 130, 145, and 155 has been demonstrated. And this complex is essential for U2 snRNP to anchor to the pre-mRNA. Splicing factor 3B subunit 2 (SF3B2), known as SAP145, forms complex with SAP49 which plays a role in cell cycle progression. SAP145, with predicated MW of 100 kDa, appears with MW of about 145 kDa which may be due to the post-translational modification.

Publications

Species	Application	Title
human	WB	Gastroenterology; Enterotoxigenic Bacteroides fragilis promotes intestinal inflammation and malignancy by inhibiting exosomes-packaged miR-149-3p.; Authors - Yingying Cao
human	WB	Nucleic Acids Res; MEN1 is a regulator of alternative splicing and prevents R-loop-induced genome instability through suppression of RNA polymerase II elongation; Authors - Bangming Jin
human	WB	Nucleic Acids Res; CUGBP2 directly interacts with U2 17S snRNP components and promotes U2 snRNA binding to cardiac troponin T pre-mRNA.; Authors - Goo Young-Hwa YH
human	WB	Front Oncol; Novel Non-Coding Transcript in NR4A3 Locus, LncNR4A3, Regulates RNA Processing Machinery Proteins and NR4A3 Expression.; Authors - Ada Congrains
human	WB	J Exp Clin Cancer Res; CircSCAP interacts with SF3A3 to inhibit the malignance of non-small cell lung cancer by activating p53 signaling.; Authors - Dongni Chen
human	IHC	Cell Rep; Spatial region-resolved proteome map reveals mechanism of COVID-19-associated heart injury.; Authors - Ling Leng