Anticorps Polyclonal de lapin anti-SETD1B

SETD1B Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ChIP, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 55005-1-AP

Synonymes

hSET1B, KIAA1076, KMT2G, Lysine N methyltransferase 2G, SET domain containing 1B, Set1B, SETD1B

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Galerie de données de validation

• 

  WB analysis of human brain using 55005-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55005-1-AP (SETD1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 55005-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 55005-1-AP (SETD1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 55005-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55005-1-AP (SETD1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 55005-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55005-1-AP (SETD1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 55005-1-AP

  IP Result of anti-SETD1B (IP:55005-1-AP, 5ug; Detection:55005-1-AP 1:300) with MCF-7 cells lysate 3250ug.

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  IHC staining of human breast cancer using 55005-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55005-1-AP (SETD1B Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary tumor using 55005-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 55005-1-AP (SETD1B Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary tumor using 55005-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 55005-1-AP (SETD1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 55005-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55005-1-AP (SETD1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 55005-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55005-1-AP (SETD1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 55005-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55005-1-AP (SETD1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 55005-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55005-1-AP (SETD1B Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary tumor using 55005-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 55005-1-AP (SETD1B Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of MCF-7 using 55005-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 55005-1-AP (SETD1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules MCF-7, tissu hépatique de souris
• Résultats positifs en IP: cellules MCF-7
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 3 publications below
• ChIP: See 2 publications below

Informations sur le produit

55005-1-AP cible SETD1B dans les applications de WB, IP, IHC, IF, ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: SET domain containing 1B
• Masse moléculaire calculée: 209 kDa
• Poids moléculaire observé: 250-260 kDa, 450 kDa
• Numéro d’acquisition GenBank: NM_015048
• Symbole du gène: SETD1B
• Identification du gène (NCBI): 23067
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SETD1B, also named as KMT2G. Set1B associates with a about 450 kDa complex that contains all five non-catalytic components of the Set1A complex, including CFP1, Rbbp5, Ash2, Wdr5, and Wdr82. The complex is a histone methyltransferase that produces trimethylated histone H3 at Lys4. Set1B is widely expressed, and localize to a largely non-overlapping set of euchromatic nuclear speckles, suggesting that Set1B bind to a unique set of target genes and thus make non-redundant contributions to the epigenetic control of chromatin structure and gene expression( PMID: 17355966). The antibody is specific to SETD1B and, in westernbloting experiments, two specific bands can be detected, about 250 kDa, 450 kDa respectively, in addition, a unknown band around 70 kDa. This antibody is a rabbit polyclonal antibody raised against a region of human SETD1B.

Publications

Species	Application	Title
human	WB	Int J Mol Sci; (20S) Ginsenoside Rh2 Exerts Its Anti-Tumor Effect by Disrupting the HSP90A-Cdc37 System in Human Liver Cancer Cells.; Authors - Chen Chen
human	WB,IHC	Am J Cancer Res; Risk model-guided identification of MTDH expression as a marker for ferroptosis induction therapy in head and neck squamous cell carcinoma; Authors - Xueying Wang
mouse	IHC	Histochem Cell Biol; Expression of the histone lysine methyltransferases SETD1B, SETDB1, SETD2, and CFP1 exhibits significant changes in the oocytes and granulosa cells of aged mouse ovaries.; Authors - Yesim Bilmez
mouse	WB,IHC,ChIP	Int Immunopharmacol; NF-κB p65 and SETDB1 expedite lipopolysaccharide-induced intestinal inflammation in mice by inducing IRF7/NLR-dependent macrophage M1 polarization; Authors - Li Chen
mouse	WB	Cell Rep; A program of successive gene expression in mouse one-cell embryos; Authors - Maki Asami
human	ChIP	Mol Cancer Res; Downregulation of BASP1 promotes temozolomide resistance in gliomas via epigenetic activation of the FBXO32/NF-κB/MGMT axis; Authors - Xinyi Liao