Anticorps Polyclonal de lapin anti-SETBP1

• Phare
• Validé par KD/KO

SETBP1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 16841-1-AP

Synonymes

KIAA0437, SEB, SET binding protein, SET binding protein 1, SETBP1

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Galerie de données de validation

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  WB analysis of HeLa using 16841-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16841-1-AP (SETBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

16841-1-AP cible SETBP1 dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SETBP1 Protéine recombinante Ag10532
• Nom complet: SET binding protein 1
• Masse moléculaire calculée: 175 kDa, 26 kDa
• Poids moléculaire observé: 170-180 kDa
• Numéro d’acquisition GenBank: BC062338
• Symbole du gène: SETBP1
• Identification du gène (NCBI): 26040
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SETBP1 is initially found to interact with SET, which is a small protein inhibitor for tumor suppressors PP2A and NM23-H1. There are existing two isoforms of SETBP1 and the molecular weight of isoform one is 170 kDa and another is 26 kDa. SET is fused with another gene CAN through chromosome translocation in a case of acute undifferentiated leukemia. SETBP1 may involve in chromosome translocation in a case of acute T-cell leukemia [PMID:17569777]. SETBP1 can cooperate with BCR/ABL to transform committed myeloid progenitors normally lacking self-renewal capability into LSCs and cause development of myeloid leukemias resembling human CML myeloid blast crisis [PMID:22566606]. SETBP1 was shown to form a complex with SET and PP2A, enhancing the stability of SET and its inhibition of PP2A.

Publications

Species	Application	Title
human	WB	Nat Genet; Somatic SETBP1 mutations in myeloid malignancies.; Authors - Makishima Hideki H
mouse	WB	Blood; Setbp1 promotes the self-renewal of murine myeloid progenitors via activation of Hoxa9 and Hoxa10.; Authors - Oakley Kevin K ; KD Validated
human	WB,IF,IP	iScience; Recruitment of MLL1 complex is essential for SETBP1 to induce myeloid transformation.; Authors - Nhu Nguyen
human,mouse,canine	WB	J Biochem; The protein level of the tumour-promoting factor SET is regulated by cell density.; Authors - Naoki Kohyanagi ; KD Validated
human	WB	Oncotarget; Myb expression is critical for myeloid leukemia development induced by Setbp1 activation.; Authors - Nhu Nguyen