Anticorps Polyclonal de lapin anti-SERPING1/C1 Inactivator

SERPING1/C1 Inactivator Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 12259-1-AP

Synonymes

C1 esterase inhibitor, C1 Inh, C1 inhibiting factor, C1IN, C1INH, C1NH, HAE1, HAE2, Plasma protease C1 inhibitor, Serpin G1, SERPING1, SERPING1/C1 Inactivator

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Galerie de données de validation

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  WB analysis using 12259-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12259-1-AP (SERPING1/C1 Inactivator antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 12259-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12259-1-AP (SERPING1/C1 Inactivator antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12259-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12259-1-AP (SERPING1/C1 Inactivator antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 12259-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12259-1-AP (SERPING1/C1 Inactivator antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12259-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12259-1-AP (SERPING1/C1 Inactivator antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 12259-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12259-1-AP (SERPING1/C1 Inactivator antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 12259-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12259-1-AP (SERPING1/C1 Inactivator antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 12259-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12259-1-AP (SERPING1/C1 Inactivator antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 12259-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12259-1-AP (SERPING1/C1 Inactivator antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 12259-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SERPING1/C1 Inactivator antibody (12259-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 12259-1-AP

  Immunofluorescent analysis of HepG2 cells using 12259-1-AP (SERPING1/C1 Inactivator antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: plasma humain, cellules HeLa, cellules HepG2, cellules L02, tissu hépatique de souris, tissu hépatique humain, tissu urinaire humain
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Informations sur le produit

12259-1-AP cible SERPING1/C1 Inactivator dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SERPING1/C1 Inactivator Protéine recombinante Ag2819
• Nom complet: serpin peptidase inhibitor, clade G (C1 inhibitor), member 1
• Masse moléculaire calculée: 500 aa, 55 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC011171
• Symbole du gène: SERPING1
• Identification du gène (NCBI): 710
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SERPING1, also known as C1 Inhibitor (C1INH), is a member of the Serine proteinase inhibitor family. Its main function is the inhibition of the complement system to prevent spontaneous activation. It inhibits activated C1r and C1s of the first complement component and thus regulates complement activation. Deficiency of this protein is associated with hereditary angioneurotic oedema (HANE). C1INH is the most heavily glycosylated plasma protein. Of its 95-105 kDa apparent molecular mass, the protein moiety of 500 aa accounts for only 55 kDa. The native 105 kDa form of C1INH could be cleaved into 60-65 kDa fragments.

Publications

Species	Application	Title
mouse	WB	Glia; Knockdown of circulating C1 inhibitor induces neurovascular impairment, glial cell activation, neuroinflammation, and behavioral deficits.; Authors - Dorit Farfara
mouse	IF	J Neuroinflammation; Fluoxetine inhibited the activation of A1 reactive astrocyte in a mouse model of major depressive disorder through astrocytic 5-HT2BR/β-arrestin2 pathway.; Authors - Yinquan Fang
human	WB	J Thromb Haemost; The Alzheimer's disease peptide Aβ promotes thrombin generation through activation of coagulation factor XII.; Authors - Daria Zamolodchikov
mouse	WB	J Thromb Haemost; The Alzheimer's disease peptide β-amyloid promotes thrombin generation through activation of coagulation factor XII: reply.; Authors - D Zamolodchikov
human	IHC	J Alzheimers Dis; A New Serum Biomarker Set to Detect Mild Cognitive Impairment and Alzheimer's Disease by Peptidome Technology.; Authors - Koji Abe
human	IHC	Urol Oncol; Decreased expression of serine protease inhibitor family G1 (SERPING1) in prostate cancer can help distinguish high-risk prostate cancer and predicts malignant progression.; Authors - Shengmeng Peng