Anticorps Monoclonal anti-Protein C inhibitor

Protein C inhibitor Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

2H6G2

N° de cat : 66030-1-Ig

Synonymes

PAI3, PCI, PLANH3, PROCI, Protein C inhibitor, SERPINA5

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Galerie de données de validation

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  WB analysis of human testis using 66030-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 66030-1-Ig (Protein C inhibitor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 66030-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 66030-1-Ig (Protein C inhibitor antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 66030-1-Ig

  human spleen tissue were subjected to SDS PAGE followed by western blot with 66030-1-Ig (Protein C inhibitor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 66030-1-Ig

  human spleen tissue were subjected to SDS PAGE followed by western blot with 66030-1-Ig (Protein C inhibitor antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 66030-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66030-1-Ig (Protein C inhibitor antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 66030-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66030-1-Ig (Protein C inhibitor antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human ovary tumor using 66030-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using Protein C inhibitor antibody (66030-1-Ig, Clone: 2H6G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human ovary tumor using 66030-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using Protein C inhibitor antibody (66030-1-Ig, Clone: 2H6G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: testicule humain, tissu
• Résultats positifs en IHC: tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de tumeur ovarienne humain,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66030-1-Ig cible Protein C inhibitor dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Protein C inhibitor Protéine recombinante Ag18094
• Nom complet: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC008915
• Symbole du gène: SERPINA5
• Identification du gène (NCBI): 5104
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SERPINA5 (also called Protein C Inhibitor, PCI ) is a serpin type of serine protease inhibitor that is found in many tissues and fluids in human, including blood plasma, seminal plasma and urine. PCI found in blood originates from the liver and is capable of inhibiting several serine proteases involved in the regulation of coagulation and fibrinolysis, including activated protein C, thrombin, factor Xa, various kallikreins and plasminogen activators. PCI has been found to have antimicrobial and antitumor properties and thus appears to be a multi-functional protein.