Anticorps Polyclonal de lapin anti-Septin 8

• Phare
• Validé par KD/KO

Septin 8 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 11769-1-AP

Synonymes

KIAA0202, SEP2, SEPT8, septin 8, septin8

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Galerie de données de validation

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  WB analysis of HeLa using 11769-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11769-1-AP (Septin 8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 11769-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11769-1-AP (Septin 8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11769-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11769-1-AP (Septin 8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11769-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11769-1-AP (Septin 8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11769-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11769-1-AP (Septin 8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11769-1-AP

  IP result of anti-Septin 8 (IP:11769-1-AP, 3ug; Detection:11769-1-AP 1:1000) with HeLa cells lysate 2000ug.

• 

  IHC staining of human gliomas using 11769-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11769-1-AP (Septin 8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11769-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11769-1-AP (SEPT8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 11769-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11769-1-AP (Septin 8 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 11769-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11769-1-AP (SEPT8 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human gliomas using 11769-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11769-1-AP (Septin 8 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11769-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11769-1-AP (SEPT8 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of hTERT-RPE1 using 11769-1-AP

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using Septin 8 antibody (11769-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules K-562, tissu cérébral de rat, tissu cérébral de souris, tissu testiculaire de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de gliome humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules hTERT-RPE1,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IF: See 5 publications below

Informations sur le produit

11769-1-AP cible Septin 8 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Septin 8 Protéine recombinante Ag2357
• Nom complet: septin 8
• Masse moléculaire calculée: 55.6 kDa
• Poids moléculaire observé: 50-56 kDa
• Numéro d’acquisition GenBank: BC001329
• Symbole du gène: Septin 8
• Identification du gène (NCBI): 23176
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Septin 8, also named as KIAA0202, is a member of the highly conserved septin family. Septins are 40-60kD GTPases that assemble as filamentous scaffolds. They are involved in the organization of submembranous structures, in neuronal polarity, and in vesicle trafficking. It may play a role in platelet secretion. Septin 8 is about 50-56 kDa in WB detection.

Publications

Species	Application	Title
mouse	WB,IF	Elife; Septin/anillin filaments scaffold central nervous system myelin to accelerate nerve conduction.; Authors - Julia Patzig
mouse	WB,IF	Thromb Haemost; Impaired Platelet Function in Sept8-Deficient Mice In Vitro; Authors - Katharina Neubauer
		Glia; The formation of paranodal spirals at the ends of CNS myelin sheaths requires the planar polarity protein Vangl2.; Authors - Andrew A Jarjour
mouse	WB	Development; Pinch2 regulates myelination in the mouse central nervous system.; Authors - Joana Paes de Faria
mouse, human	WB	J Cell Sci; SEPT8 modulates β-amyloidogenic processing of APP by affecting the sorting and accumulation of BACE1.; Authors - Kaisa M A Kurkinen ; KD Validated
mouse	IF	Cytoskeleton (Hoboken); Characterization of septin expression in normal and fibrotic kidneys.; Authors - Katharina Neubauer