Anticorps Polyclonal de lapin anti-Septin 4

• Phare
• Validé par KD/KO

Septin 4 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (1)

N° de cat : 12476-1-AP

Synonymes

ARTS, BRADEION, Bradeion beta, Brain protein H5, CE5B3, CE5B3 beta, Cerebral protein 7, H5, hCDCREL 2, hucep 7, MART, Peanut like protein 2, PNUTL2, SEP4, SEPT4, septin 4, Septin4

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Galerie de données de validation

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  WB analysis using 12476-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12476-1-AP (Septin 4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 12476-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12476-1-AP (Septin 4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of A375 using 12476-1-AP

  IP result of anti-Septin 4 (IP:12476-1-AP, 4ug; Detection:12476-1-AP 1:300) with A375 cells lysate 2200ug.

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  IHC staining of mouse brain using 12476-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12476-1-AP (Septin 4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12476-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12476-1-AP (Septin 4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of hTERT-RPE1 using 12476-1-AP

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using Septin 4 antibody (12476-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules A375, tissu cérébral de rat, tissu de cervelet de souris
• Résultats positifs en IP: cellules A375
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules hTERT-RPE1,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

12476-1-AP cible Septin 4 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Septin 4 Protéine recombinante Ag3185
• Nom complet: septin 4
• Masse moléculaire calculée: 459 aa, 53 kDa
• Poids moléculaire observé: 54 kDa, 52 kDa, 48 kDa, 44 kDa
• Numéro d’acquisition GenBank: BC018056
• Symbole du gène: SEPT4
• Identification du gène (NCBI): 5414
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Spetin 4 is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Spetin 4 was widely expressed in brain and heart, also expressed in colorectal cancers and malignant melanomas. This protein has been implicated in diverse cellular functions, including cytokinesis, apoptosis, and tumor suppression(PMID:15737931). There are several isoforms of SEPTIN4, four of which were detected in the mouse brain and mouse cerebellum, 54 kDa, 52 kDa, 48 kDa and 44 kDa respectively(PMID: 23938054; PMID: 26657011).

Publications

Species	Application	Title
human	WB	Bioorg Chem; Discovery of a septin-4 covalent binder with antimetastatic activity in a mouse model of melanoma; Authors - Federica Blua
human	WB	Int J Biol Sci; Septin4 Prevents PDGF-BB-induced HAVSMC Phenotypic Transformation, Proliferation and Migration by Promoting SIRT1-STAT3 Deacetylation and Dephosphorylation.; Authors - Naijin Zhang ; KD Validated
human	WB,IF,IP	Biochem Biophys Res Commun; Septin4 as a novel binding partner of PARP1 contributes to oxidative stress induced human umbilical vein endothelial cells injure.; Authors - Naijin Zhang ; KD Validated
human,mouse	IF,CoIP	Front Endocrinol (Lausanne); A novel mutation in CFAP47 causes male infertility due to multiple morphological abnormalities of the sperm flagella; Authors - Mohan Liu
mouse	WB	Biomed Pharmacother; Atorvastatin rescues vascular endothelial injury in hypertension by WWP2-mediated ubiquitination and degradation of ATP5A; Authors - Zeyu Yin

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Mohammed (Verified Customer) (08-19-2020)

Septin 4 stained the annulus of sperm very beautifully. Very little background and a nice sharp stain.

• Applications: Immunofluorescence
• Primary Antibody Dilution: (1:200)
• Cell Tissue Type: Testis