Anticorps Polyclonal de lapin anti-Septin 2

• Phare
• Validé par KD/KO

Septin 2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

24

Avis (2)

N° de cat : 11397-1-AP

Synonymes

NEDD-5, NEDD5, NEDD 5, KIAA0158, hNedd5

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Galerie de données de validation

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  WB analysis using 11397-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11397-1-AP (Septin 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11397-1-AP

  WB result of Septin 2 antibody (11397-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Septin 2 transfected HEK-293 cells.

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  WB analysis of HeLa using 11397-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11397-1-AP (Septin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 11397-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11397-1-AP (Septin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 11397-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 11397-1-AP (Septin 2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 11397-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11397-1-AP (Septin 2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 11397-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11397-1-AP (Septin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11397-1-AP

  IP result of anti-Septin 2 (IP:11397-1-AP, 3ug; Detection:11397-1-AP 1:1000) with mouse brain tissue lysate 5000ug.

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  IHC staining of human liver cancer using 11397-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11397-1-AP (Septin 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 11397-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11397-1-AP (Septin 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 11397-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11397-1-AP (Septin 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 11397-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11397-1-AP (Septin 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11397-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 11397-1-AP (Septin 2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 11397-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Septin 2 antibody (11397-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of hTERT-RPE1 cells and Mouse embryonic fibroblasts using 11397-1-AP

  IF result from Dr. Kevin Corbit. Septin 2 (11397-1-AP) marks the transition zone and cilium of Human hTERT-RPE1 cells and Mouse embryonic fibroblasts.

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  FC experiment of MCF-7 using 11397-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human Septin 2 (11397-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules C6, cellules HEK-293, cellules HeLa, cellules Jurkat, cellules K-562, tissu cardiaque de souris, tissu cérébral de rat, tissu de muscle squelettique humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules hTERT-RPE1 et fibroblastes embryonnaires de souris
• Résultats positifs en FC (Intra): cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 16 publications below
• IF: See 13 publications below
• IP: See 1 publications below

Informations sur le produit

11397-1-AP cible Septin 2 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Septin 2 Protéine recombinante Ag1946
• Nom complet: septin 2
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC014455
• Symbole du gène: Septin 2
• Identification du gène (NCBI): 4735
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SEPT2 belongs to a family of septins, GTP-binding proteins that are a component of the cytoskeleton. Septins can assemble into intracellular structures of various shapes and are known to influence membrane stability, forming diffusion barriers and serving as scaffolds.

What is the molecular weight of SEPT2? Is SEPT2 post-translationally modified?

The molecular weight of SEPT2 is 45 kDa. In a cellular context, SEPT2 forms hetero-hexamers and hetero-octamers with other septins. These hexamers and octamers further assemble into high-order structures such as filaments and rings. SEPT2 can be phosphorylated on Ser218 by casein kinase 2 (PMID: 19165576) and can also be SUMOylated (PMID: 29051266).

What is the subcellular localization of SEPT2?

SEPT2 forms a diffusion barrier and localizes to the primary cilium; in some cell types, it has been found at the transition zone/basal body (PMID: 20558667), while in others localized to the axoneme (PMID: 23572511). During cell division, SEPT2 accumulates near the contractile ring from anaphase through telophase, and finally condenses into the midbody (PMID: 9203580). In interphase cells, SEPT2 was found to associate with polyglutamylated microtubules (PMID: 18209106) and can affect microtubule dynamics via interaction with MAP4.

What is the tissue expression pattern of SEPT2?

While some septins have tissue-specific expression profiles, SEPT2 is ubiquitously expressed (PMID: 22314400).

Publications

Species	Application	Title
human	IF	Nat Commun; Wiskott-Aldrich syndrome protein regulates autophagy and inflammasome activity in innate immune cells.; Authors - Pamela P Lee
human	WB,IF	J Cell Biol; The septin cytoskeleton regulates natural killer cell lytic granule release.; Authors - Prasad V Phatarpekar
mouse	WB,IF	PLoS Genet; Genetic deletion of SEPT7 reveals a cell type-specific role of septins in microtubule destabilization for the completion of cytokinesis.; Authors - Manoj B Menon
human		Cell Death Dis; JMJD2C-mediated long non-coding RNA MALAT1/microRNA-503-5p/SEPT2 axis worsens non-small cell lung cancer.; Authors - Jun Zhang
mouse	WB	Elife; Septin/anillin filaments scaffold central nervous system myelin to accelerate nerve conduction.; Authors - Julia Patzig
mouse	IF	PLoS Genet; SEPT12 phosphorylation results in loss of the septin ring/sperm annulus, defective sperm motility and poor male fertility.; Authors - Yi-Ru Shen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Christine (Verified Customer) (01-27-2023)

Incubation for a few hours at room temperature on HEK293T lysates. Detection of a very strong signal just below the 47 kDa marker (expected size of Septin 2) and a fainter signal just above the 47 kDa marker.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

FH

Mohammed (Verified Customer) (08-19-2020)

Some staining at the annulus, there seems to be staining in the acrosome. Overall, really high background (even when the concentration is lowered).

• Applications: Immunofluorescence
• Primary Antibody Dilution: (1:200)
• Cell Tissue Type: Testis