Anticorps Polyclonal de lapin anti-SENP5-Specific

• Phare
• Validé par KD/KO

SENP5-Specific Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 19529-1-AP

Synonymes

SENP5, SENP5-Specific, Sentrin specific protease 5

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Galerie de données de validation

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  WB analysis using 19529-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19529-1-AP (SENP5-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 19529-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19529-1-AP (SENP5-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 19529-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19529-1-AP (SENP5-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 19529-1-AP

  IP result of anti-SENP5-Specific (IP:19529-1-AP, 4ug; Detection:19529-1-AP 1:300) with HeLa cells lysate 520ug.

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  IHC staining of human cervical cancer using 19529-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19529-1-AP (SENP5-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 19529-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19529-1-AP (SENP5-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 19529-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19529-1-AP (SENP5-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cervical cancer using 19529-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19529-1-AP (SENP5-Specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 19529-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 19529-1-AP (SENP5-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 19529-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 19529-1-AP (SENP5-Specific antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, tissu hépatique de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

19529-1-AP cible SENP5-Specific dans les applications de WB, IP, IF, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: SUMO1/sentrin specific peptidase 5
• Masse moléculaire calculée: 87 kDa
• Poids moléculaire observé: 97 kDa
• Numéro d’acquisition GenBank: NM_152699
• Symbole du gène: SENP5
• Identification du gène (NCBI): 205564
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SENP5, also named as FKSG45, belongs to the peptidase C48 family. SENP5 is a protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO3 to its mature form and deconjugation of SUMO2 and SUMO3 from targeted proteins. SENP5 has weak proteolytic activity against full-length SUMO1 or SUMO1 conjugates. It is required for cell division. The antibody is specific to SENP5.

Publications

Species	Application	Title
	WB	EMBO Rep; The SUMO protease SENP3 regulates mitochondrial autophagy mediated by Fis1.; Authors - Emily Waters
human	WB	J Cell Sci; SUMO proteases SENP3 and SENP5 spatiotemporally regulate the kinase activity of Aurora A.; Authors - Bin Yu
human	WB	J Cell Sci; SUMO proteases SENP3 and SENP5 spatiotemporally regulate the kinase activity of Aurora A.; Authors - Bin Yu
human,mouse	WB	iScience; Drp1 SUMO/deSUMOylation by Senp5 isoforms influences ER tubulation and mitochondrial dynamics to regulate brain development.; Authors - Seiya Yamada ; KD Validated
mouse	WB,IHC,IF	J Comp Neurol; Synaptic localization of the SUMOylation-regulating protease SENP5 in the adult mouse brain.; Authors - Hiroki Akiyama ; KD Validated
mouse	WB	Cell Rep; SENP7 deSUMOylase-governed transcriptional program coordinates sarcomere assembly and is targeted in muscle atrophy; Authors - Mamta Amrute-Nayak