Anticorps Polyclonal de lapin anti-SELS

• Phare
• Validé par KD/KO

SELS Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

24

N° de cat : 15591-1-AP

Synonymes

AD 015, ADO15, SBBI8, selenoprotein S, SELS, SEPS1, VIMP

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Galerie de données de validation

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  WB analysis of rat brain using 15591-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 15591-1-AP (SELS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15591-1-AP

  WB result of SELS antibody (15591-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SELS transfected HeLa cells.

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  WB analysis of mouse brain using 15591-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15591-1-AP (SELS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 15591-1-AP

  IP result of anti-SELS (IP:15591-1-AP, 3ug; Detection:15591-1-AP 1:500) with HepG2 cells lysate 600ug.

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Applications testées

• Résultats positifs en WB: tissu cérébral de rat, cellules HeLa
• Résultats positifs en IP: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 23 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

15591-1-AP cible SELS dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SELS Protéine recombinante Ag7928
• Nom complet: selenoprotein S
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC005840
• Symbole du gène: SELS
• Identification du gène (NCBI): 55829
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SELS, also named as VIMP and SEPS1, plays an important role in the production of inflammatory cytokines and its expression is activated by endoplasmic reticulum (ER) stress. It probably acts by serving as a linker between DERL1 and VCP.

Publications

Species	Application	Title
human	WB	Sci Adv; Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation, leading to impaired CD8+ T cell response in tumor.; Authors - Yuan Wang
pig	WB	J Anim Sci Biotechnol; Selenogenome and AMPK signal insight into the protective effect of dietary selenium on chronic heat stress-induced hepatic metabolic disorder in growing pigs.; Authors - Yan Liu
human	WB	Antioxidants (Basel); Dietary Marginal and Excess Selenium Increased Triglycerides Deposition, Induced Endoplasmic Reticulum Stress and Differentially Influenced Selenoproteins Expression in the Anterior and Middle Intestines of Yellow Catfish Pelteobagrus fulvidraco.; Authors - Dian-Guang Zhang
mouse	WB	Antioxidants (Basel); Reversal of Lipid Metabolism Dysregulation by Selenium and Folic Acid Co-Supplementation to Mitigate Pathology in Alzheimer's Disease.; Authors - Zhong-Hao Zhang
pig	WB	Antioxidants (Basel); Hydroxy Selenomethionine Improves Meat Quality through Optimal Skeletal Metabolism and Functions of Selenoproteins of Pigs under Chronic Heat Stress.; Authors - Yan Liu
pig	WB	Antioxidants (Basel); Hydroxy Selenomethionine Alleviates Hepatic Lipid Metabolism Disorder of Pigs Induced by Dietary Oxidative Stress via Relieving the Endoplasmic Reticulum Stress.; Authors - Jinzhong Jing