Anticorps Polyclonal de lapin anti-SEC63

• Phare
• Validé par KD/KO

SEC63 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 13978-1-AP

Synonymes

Translocation protein SEC63 homolog, SEC63L, DNAJC23, DnaJ homolog subfamily C member 23

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Galerie de données de validation

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  WB analysis of A431 using 13978-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 13978-1-AP (SEC63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 13978-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13978-1-AP (SEC63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13978-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13978-1-AP (SEC63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 13978-1-AP

  IP result of anti-SEC63 (IP:13978-1-AP, 4ug; Detection:13978-1-AP 1:300) with A431 cells lysate 2400ug.

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  IHC staining of human liver using 13978-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13978-1-AP (SEC63 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 13978-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13978-1-AP (SEC63 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 13978-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 13978-1-AP (SEC63 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 13978-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 13978-1-AP (SEC63 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse kidney using 13978-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using 13978-1-AP (SEC63 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse kidney using 13978-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using 13978-1-AP (SEC63 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 13978-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SEC63 antibody (13978-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules PC-3
• Résultats positifs en IP: cellules A431
• Résultats positifs en IHC: tissu hépatique humain, tissu pancréatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu rénal de souris,
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

13978-1-AP cible SEC63 dans les applications de WB, IHC, IF/ICC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SEC63 Protéine recombinante Ag5021
• Nom complet: SEC63 homolog (S. cerevisiae)
• Masse moléculaire calculée: 88 kDa
• Poids moléculaire observé: 83-90 kDa
• Numéro d’acquisition GenBank: BC047221
• Symbole du gène: SEC63
• Identification du gène (NCBI): 11231
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SEC63 is an integral ER membrane protein. SEC63 and SEC62 form a dimeric complex and play a central role in the translocation of nascent and newly synthesized precursor polypeptides into the ER. Mutations in the gene encoding SEC63 cause polycystic liver disease in humans. The intact SEC63 protein is usually detected around 83-90 kDa, while smaller cleavage products can be observed by autophagy proteolysis (PMID: 25052096)

Publications

Species	Application	Title
	WB	Mol Cell; Cotranslational Stabilization of Sec62/63 within the ER Sec61 Translocon Is Controlled by Distinct Substrate-Driven Translocation Events.; Authors - Brian J Conti
mouse	WB	J Clin Invest; Sec63 and Xbp1 regulate IRE1α activity and polycystic disease severity.; Authors - Sorin V Fedeles
mouse	WB, IF	J Am Soc Nephrol; Essential Role of X-Box Binding Protein-1 during Endoplasmic Reticulum Stress in Podocytes.; Authors - Hossam Hassan ; KO Validated
human	WB	J Biol Chem; ER entry pathway and glycosylation of GPI-anchored proteins are determined by N-terminal signal sequence and C-terminal GPI-attachment sequence; Authors - Tetsuya Hirata ; KO Validated