Anticorps Polyclonal de lapin anti-SDCCAG3

• Phare
• Validé par KD/KO

SDCCAG3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 15969-1-AP

Synonymes

SDCCAG 3, NY CO 3, ENTR1, Antigen NY-CO-3

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Galerie de données de validation

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  WB analysis of HeLa using 15969-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15969-1-AP (SDCCAG3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 15969-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15969-1-AP (SDCCAG3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 15969-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15969-1-AP (SDCCAG3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 15969-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15969-1-AP (SDCCAG3 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 15969-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SDCCAG3 antibody (15969-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  FC experiment of HepG2 using 15969-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug SDCCAG3 Polyclonal antibody (15969-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 7 publications below

Informations sur le produit

15969-1-AP cible SDCCAG3 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SDCCAG3 Protéine recombinante Ag8745
• Nom complet: serologically defined colon cancer antigen 3
• Masse moléculaire calculée: 412 aa, 46 kDa
• Poids moléculaire observé: 48-60 kDa
• Numéro d’acquisition GenBank: BC014515
• Symbole du gène: SDCCAG3
• Identification du gène (NCBI): 10807
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SDCCAG3, also named as NY-CO-3, is identified as serologically defined colon cancer antigen-3. It is an endosomal protein which is involved in the regulation of cytokinesis. SDCCAG3 is involved in modulation of TNF response. This gene has four isoforms with MW 40-50 kDa. Observed MW this protein is 48-60 kDa may due to phosphorylation.

Publications

Species	Application	Title
human	IF	Nat Commun; Apoptotic signalling targets the post-endocytic sorting machinery of the death receptor Fas/CD95.; Authors - Shruti Sharma
human	WB,IF	Curr Biol; Retromer Binding to FAM21 and the WASH Complex Is Perturbed by the Parkinson Disease-Linked VPS35(D620N) Mutation.; Authors - Ian J McGough
human	WB,IF	Oncogene; The serologically defined colon cancer antigen-3 interacts with the protein tyrosine phosphatase PTPN13 and is involved in the regulation of cytokinesis.; Authors - Hagemann N N ; KD Validated
human	WB, IF	J Cell Sci; Identification of molecular heterogeneity in SNX27-retromer-mediated endosome-to-plasma membrane recycling.; Authors - Ian J McGough ; KD Validated
human	WB,IF	Sci Rep; The serologically defined colon cancer antigen-3 (SDCCAG3) is involved in the regulation of ciliogenesis.; Authors - Fangyan Yu ; KD Validated
rat	IF	J Dent Res; Sdccag3 Promotes Implant Osseointegration during Experimental Hyperlipidemia.; Authors - H Ren