Anticorps Polyclonal de lapin anti-SDC4

• Phare
• Validé par KD/KO

SDC4 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 11820-1-AP

Synonymes

Amphiglycan, Ryudocan core protein, SDC4, SYND4, syndecan 4

---

Galerie de données de validation

• 

  WB analysis using 11820-1-AP

  NIH/3T3 lysates were subjected to SDS PAGE followed by western blot with 11820-1-AP (SDC4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse skeletal muscle using 11820-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11820-1-AP (SDC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 11820-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11820-1-AP (SDC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11820-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11820-1-AP (SDC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11820-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11820-1-AP (SDC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11820-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11820-1-AP (SDC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11820-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11820-1-AP (SDC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules NIH/3T3,
• Résultats positifs en IHC: tissu de muscle squelettique de souris, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Informations sur le produit

11820-1-AP cible SDC4 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SDC4 Protéine recombinante Ag2388
• Nom complet: syndecan 4
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22-26 kDa
• Numéro d’acquisition GenBank: BC030805
• Symbole du gène: SDC4
• Identification du gène (NCBI): 6385
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SDC4, also named as syndecan 4, is a cell surface proteoglycan that bears heparan sulfate, which is involved in normal wound healing. It has been reported that SDC4may be associated with keratinocyte proliferation and differentiation in the epidermis. SDC4 also plays a key role in the angiogenesis and inflammation. The predicted MW of SDC4 is 22 kDa and 11820-1-AP antibody recognizes a protein around 24-26 kDa and 48-52 kDa (homodimer) which is similar to the papers.(PMID: 20639201, 21949132; 18638267; 30165731 )

Publications

Species	Application	Title
rat	WB,IF	Front Pharmacol; Dioscin Inhibits HSC-T6 Cell Migration via Adjusting SDC-4 Expression: Insights from iTRAQ-Based Quantitative Proteomics.; Authors - Lianhong Yin
human	WB	Oncol Lett; Cadherin-16 inhibits thyroid carcinoma cell proliferation and invasion.; Authors - Xiaolin Yang
human	WB,IF,IHC	Cell Death Dis; Therapeutic potential of targeting membrane-spanning proteoglycan SDC4 in hepatocellular carcinoma.; Authors - Heng Yang ; KD Validated
human	WB	eBioMedicine; Atypical E3 ligase ZFP91 promotes small-molecule-induced E2F2 transcription factor degradation for cancer therapy; Authors - Ting-Ting Liu
mouse	IHC,IF	Front Pediatr; Bioinformatics analysis identifies heparan sulfate proteoglycans acting as different progress subtypes of biliary atresia; Authors - Zequan Ding
human	IHC	EBioMedicine; Spatial and single-cell colocalisation analysis reveals MDK-mediated immunosuppressive environment with regulatory T cells in colorectal carcinogenesis; Authors - Masahiro Hashimoto