Anticorps Polyclonal de lapin anti-SCUBE3

SCUBE3 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (1)

N° de cat : 16773-1-AP

Synonymes

CEGF3, SCUBE3

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Galerie de données de validation

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  WB analysis of mouse kidney using 16773-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16773-1-AP (SCUBE3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 16773-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16773-1-AP (SCUBE3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 16773-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16773-1-AP (SCUBE3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 16773-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16773-1-AP (SCUBE3 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, tissu pulmonaire de souris
• Résultats positifs en IHC: tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

16773-1-AP cible SCUBE3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SCUBE3 Protéine recombinante Ag10085
• Nom complet: signal peptide, CUB domain, EGF-like 3
• Masse moléculaire calculée: 992 aa, 109 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC052263
• Symbole du gène: SCUBE3
• Identification du gène (NCBI): 222663
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SCUBE3 is a secreted glycoprotein that belongs to the SCUBE family. Members of the SCUBE family are secreted plasma membrane-associated proteins characterised by a N-terminal signal peptide sequence, multiple EGF domains, a N-linked glycosylated spacer region and a C-terminal CUB region. SCUBE3 may play a role in bone cell biology (PMID: 15234972). It may also has a role in the regulation of cardiac growth and remodeling responses (PMID: 17442284). Besides, SCUBE3 has been reported to be involved in lung cancer progression (PMID: 21441952; 23420440).

Publications

Species	Application	Title
human	IHC	Gene; Comprehensive and in-depth analysis of microRNA and mRNA expression profile in salivary adenoid cystic carcinoma.; Authors - Nannan Han
human,mouse	WB	Mol Cell Endocrinol; β-catenin/TCF4-induced SCUBE3 upregulation promotes ovarian cancer development via HIF-1 signaling pathway; Authors - Jing Shen ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (10-04-2021)

Calculated band is 109 kd. This antibody recognized a major band at 60 kd. There was a very weak band ~130 kd, but was PNGaseF insensitive, suggesting this band is not a glycosylated form of this protein.

• Applications: Western Blot