Anticorps Polyclonal de lapin anti-SCRN3

SCRN3 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 13240-1-AP

Synonymes

SCRN3, secernin 3, SES3

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Galerie de données de validation

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  WB analysis of HepG2 using 13240-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13240-1-AP (SCRN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13240-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13240-1-AP (SCRN3 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 13240-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13240-1-AP (SCRN3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 13240-1-AP

  IP result of anti-SCRN3 (IP:13240-1-AP, 4ug; Detection:13240-1-AP 1:800) with HEK-293 cells lysate 1760ug.

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  IHC staining of human pancreas using 13240-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13240-1-AP (SCRN3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 13240-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13240-1-AP (SCRN3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas using 13240-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13240-1-AP (SCRN3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 13240-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13240-1-AP (SCRN3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 13240-1-AP

  Immunofluorescent analysis of HepG2 cells using 13240-1-AP (SCRN3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A549, cellules HEK-293
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu rénal humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

13240-1-AP cible SCRN3 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SCRN3 Protéine recombinante Ag3777
• Nom complet: secernin 3
• Masse moléculaire calculée: 424 aa, 49 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC031821
• Symbole du gène: SCRN3
• Identification du gène (NCBI): 79634
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SCRN genes encode protein members of the secernin family including Secernin 1, Secernin 2 and Secernin 3. Function of Secernin 2 and Secernin 3 are so far not well understood. Secernin 1 is a novel 50-kDa cytosolic protein that appears to be involved in the regulation of exocytosis from peritoneal mast cells. Secernin-1 a novel tumor-associated antigen (TAA) and may be a universal marker of different cancer types including gastric cancer, which has been validated by several researches. Measurement of SCRN1 can be used in the early detection of cancer or in the surveillance of patients who undergo surgery.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kwan Ho (Verified Customer) (05-13-2022)

This Ab can detect endogenous SCRN3 in HEK293T cells, which was validated with KO cells. However, it also binds to off-targets.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T cells