Anticorps Polyclonal de lapin anti-SCP2/SCPx

SCP2/SCPx Polyclonal Antibody for ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 19182-1-AP

Synonymes

NLTP, NSL TP, SCP 2, SCP chi, SCP X, SCP2, sterol carrier protein 2, Sterol carrier protein X

---

Galerie de données de validation

• 

  WB analysis of A2780 using 19182-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 19182-1-AP (SCPx antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 19182-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19182-1-AP (SCPx antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 19182-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 19182-1-AP (SCPx antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

---

Applications testées

Applications publiées

• WB: See 4 publications below

Informations sur le produit

19182-1-AP cible SCP2/SCPx dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SCP2/SCPx Protéine recombinante Ag6002
• Nom complet: sterol carrier protein 2
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 46 kDa, 58 kDa
• Numéro d’acquisition GenBank: BC067108
• Symbole du gène: SCP2
• Identification du gène (NCBI): 6342
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SCP2 (Sterol carrier protein-2), also called nonspecific lipid-transfer protein, is thought to play a major role in intracellular lipid transport and metabolism. Mutations of SCP2 have been associated with diseases involving abnormalities in lipid trafficking, such as Zellweger syndrome. The alternative splicing generates 58 kDa SCPx and 13-15 kDa SCP2 proteins, both of which contain a C-terninal SCP2 domain. In addition to the SCP2 domain, SCPx also contains an amino-terminal thiolase domain. The proteolytic cleavage of SCPx occurred when it was imported into peroxisomes, giving rise to a 44-46 kDa thiolase and a 13-15 kDa SCP2. 19182-1-AP antibody recognizes the intact 58 kDa SCPx protein as well as 44-46 kDa thiolase.

Publications

Species	Application	Title
	WB	J Biol Chem; A Cargo-centered Perspective on the PEX5 Receptor-mediated Peroxisomal Protein Import Pathway.; Authors - Francisco Tania T
	WB	FEBS J; Membrane topologies of PEX13 and PEX14 provide new insights on the mechanism of protein import into peroxisomes.; Authors - Aurora Barros-Barbosa
rat	WB	J Biol Chem; The peroxisomal matrix protein translocon is a large cavity-forming protein assembly into which PEX5 protein enters to release its cargo.; Authors - Ana F Dias
	WB	Methods Mol Biol; Determining the Topology of Peroxisomal Proteins Using Protease Protection Assays.; Authors - Tânia Francisco