Anticorps Polyclonal de lapin anti-RPS4X

• Phare
• Validé par KD/KO

RPS4X Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA, PLA

Conjugaison

Non conjugué

Publications

11

N° de cat : 14799-1-AP

Synonymes

CCG2, DXS306, ribosomal protein S4, X linked, RPS4, RPS4X, SCAR, SCR10

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Galerie de données de validation

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  WB analysis of HeLa using 14799-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14799-1-AP (RPS4X antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14799-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14799-1-AP (RPS4X antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14799-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14799-1-AP (RPS4X antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 14799-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14799-1-AP (RPS4X antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 14799-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14799-1-AP (RPS4X antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 14799-1-AP

  IP result of anti-RPS4X (IP:14799-1-AP, 3ug; Detection:14799-1-AP 1:500) with HepG2 cells lysate 3200ug.

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  IHC staining of human pancreas cancer using 14799-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14799-1-AP (RPS4X antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas cancer using 14799-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14799-1-AP (RPS4X antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of MCF-7 using 14799-1-AP

  Immunofluorescent analysis of MCF-7 cells, using RPS4X antibody 14799-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules MCF-7, tissu hépatique humain, tissu pancréatique de souris
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

14799-1-AP cible RPS4X dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA, PLA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris, Grenouille, xénope
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RPS4X Protéine recombinante Ag6513
• Nom complet: ribosomal protein S4, X-linked
• Masse moléculaire calculée: 263 aa, 30 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC000472
• Symbole du gène: RPS4X
• Identification du gène (NCBI): 6191
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RPS4X, a X-linked gene in the human, encodes a subunit of ribosomal protein S4, a component of the 40S subunit and 60S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene, RPS4Y2 and the ribosomal protein S4, X-linked (RPS4X). [PMID:8358435]

Publications

Species	Application	Title
mouse	WB	Cell; Regnase-1 and Roquin Regulate a Common Element in Inflammatory mRNAs by Spatiotemporally Distinct Mechanisms.; Authors - Takashi Mino
mouse	WB	Nat Commun; Excess ribosomal protein production unbalances translation in a model of Fragile X Syndrome.; Authors - Sang S Seo
Xenopus	WB, PLA	Elife; Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons.; Authors - Max Koppers
human	IHC	Aging (Albany NY); Construction and external validation of a 5-gene random forest model to diagnose non-obstructive azoospermia based on the single-cell RNA sequencing of testicular tissue; Authors - Ranran Zhou
human	WB,IHC	BMC Cancer; Low expression of the X-linked ribosomal protein S4 in human serous epithelial ovarian cancer is associated with a poor prognosis.; Authors - Tsofack Serges P SP ; KD Validated
		Biomark Med; Low level of the X-linked ribosomal protein S4 in human urothelial carcinomas is associated with a poor prognosis.; Authors - Éric R Paquet