Anticorps Polyclonal de lapin anti-SC65

• Phare
• Validé par KD/KO

SC65 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 15288-1-AP

Synonymes

NOL55, Nucleolar autoantigen No55, P3H4, SC65

---

Galerie de données de validation

• 

  WB analysis of HeLa using 15288-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15288-1-AP (SC65 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15288-1-AP

  WB result of SC65 antibody (15288-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SC65 transfected HeLa cells.

• 

  WB analysis using 15288-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15288-1-AP (SC65 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse bladder using 15288-1-AP

  IP result of anti-SC65 (IP:15288-1-AP, 4ug; Detection:15288-1-AP 1:500) with mouse bladder tissue lysate 3200ug.

---

Applications testées

• Résultats positifs en WB: cellules HeLa,
• Résultats positifs en IP: tissu vésical de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

15288-1-AP cible SC65 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SC65 Protéine recombinante Ag7480
• Nom complet: synaptonemal complex protein SC65
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC001047
• Symbole du gène: SC65
• Identification du gène (NCBI): 10609
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SC65, also named as Leprecan-like protein 4 or Nucleolar autoantigen No55, is a 437 amino acid protein, which belongs to the leprecan family. SC65 ia a protein of the interphase nucleolus and tumour-associated autoantigen in patients with prostate cancer.

Publications

Species	Application	Title
mouse	WB,IF	PLoS Genet; Sc65-Null Mice Provide Evidence for a Novel Endoplasmic Reticulum Complex Regulating Collagen Lysyl Hydroxylation.; Authors - Melissa E Heard ; KO Validated
human, mouse	WB,IF	J Bone Miner Res; Sc65 is a novel endoplasmic reticulum protein that regulates bone mass homeostasis.; Authors - Gruenwald Katrin K ; KD Validated
human	WB	Cancers (Basel); P3H4 Promotes Malignant Progression of Lung Adenocarcinoma via Interaction with EGFR.; Authors - Chen Fang ; KD Validated
mouse	WB	PLoS Genet; Cyclophilin B control of lysine post-translational modifications of skin type I collagen.; Authors - Masahiko Terajima
human	WB,IHC	Aging (Albany NY); Knockdown of P3H4 inhibits proliferation and invasion of bladder cancer.; Authors - Lin Hao ; KD Validated
human	WB	Sci Rep; Transcriptome analysis of a dog model of congestive heart failure shows that collagen-related 2-oxoglutarate-dependent dioxygenases contribute to heart failure; Authors - Takahiro Isono