Anticorps Polyclonal de lapin anti-SART3

SART3 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 18025-1-AP

Synonymes

DSAP1, hSART 3, KIAA0156, P100, p110, p110(nrb), RP11 13G14, SART 3, SART3, TIP110

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Galerie de données de validation

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  WB analysis of Jurkat using 18025-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18025-1-AP (SART3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 18025-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18025-1-AP (SART3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 18025-1-AP

  IP result of anti-SART3 (IP:18025-1-AP, 4ug; Detection:18025-1-AP 1:700) with HeLa cells lysate 2000ug.

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  IHC staining of human testis using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung cancer using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 18025-1-AP (SART3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 18025-1-AP (SART3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 18025-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 18025-1-AP (SART3 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Jurkat,
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu testiculaire humain, tissu cérébral humain, tissu cutané humain, tissu de cancer du poumon humain, tissu hépatique humain, tissu ovarien humain, tissu placentaire humain, tissu rénal humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• IP: See 2 publications below

Informations sur le produit

18025-1-AP cible SART3 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SART3 Protéine recombinante Ag12590
• Nom complet: squamous cell carcinoma antigen recognized by T cells 3
• Masse moléculaire calculée: 963 aa, 110 kDa
• Poids moléculaire observé: 110-120 kDa
• Numéro d’acquisition GenBank: BC093784
• Symbole du gène: SART3
• Identification du gène (NCBI): 9733
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The process of mRNA splicing required assembly of pre-mRNA into a large, dynamic RNA-protein complex, in which 5 small nuclear RNAs and more than 50 protein components are incorporated into functional small nuclear ribonucleoproteins(snRNPs). SART3 interacts with U6 snRNA and functioned as a U6-specific recycling factor responsible for regeneration of base-paired U4/U6 snRNPs from post-spliceosomal free U4 and U6 snRNPs. Also it can interact and regulate Tat transactivation activity, and act as a cellular factor for HIV-1 gene expression and viral replication.

Publications

Species	Application	Title
human,mouse	IP	RNA; 7SL RNA in Vertebrate Red Blood Cells.; Authors - Gaëlle J S Talhouarne
human	WB,IP	J Biol Chem; Identification of a 35S U4/U6.U5 Tri-snRNP Complex Intermediate in Spliceosome Assembly.; Authors - Zhe Chen
		Mol Cell Biol; USP15 deubiquitinates TUT1 associated with RNA metabolism and maintains cerebellar homeostasis.; Authors - Jaehyun Kim
human	IHC	Aging (Albany NY); SART3, regulated by p53, is a biomarker for diagnosis, prognosis and immune infiltration in hepatocellular carcinoma; Authors - Jusen Nong