Anticorps Polyclonal de lapin anti-SAR1A

• Phare
• Validé par KD/KO

SAR1A Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 22291-1-AP

Synonymes

COPII associated small GTPase, GTP binding protein SAR1a, masra2, SAR1, SAR1 homolog A (S. cerevisiae), SAR1A, Sara, SARA1

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Galerie de données de validation

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  WB analysis of HepG2 using 22291-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22291-1-AP (SAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 22291-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22291-1-AP (SAR1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 22291-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 22291-1-AP (SAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 22291-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 22291-1-AP (SAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 22291-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22291-1-AP (SAR1A Antibody) at dilution of 1:50.

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  IHC staining of mouse skeletal muscle using 22291-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22291-1-AP (SAR1A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 22291-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SAR1A antibody (22291-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of A431 using 22291-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using SAR1A antibody (22291-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293T, cellules HeLa, cellules HepG2, cellules HT-1080, tissu rénal de souris
• Résultats positifs en IHC: tissu de muscle squelettique de souris, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

22291-1-AP cible SAR1A dans les applications de WB, IHC, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: SAR1 homolog A (S. cerevisiae)
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 23-25 kDa
• Numéro d’acquisition GenBank: NM_020150
• Symbole du gène: SAR1A
• Identification du gène (NCBI): 56681
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SAR1A, also named as SAR1, SARA and SARA1, belongs to the small GTPase superfamily and SAR1 family. It is involved in transport from the endoplasmic reticulum to the Golgi apparatus. SAR1A is required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES). The antibody is specific to SAR1A.

Publications

Species	Application	Title
human	WB	Nature; SAR1B senses leucine levels to regulate mTORC1 signalling.; Authors - Jie Chen ; KO Validated
human	CoIP	J Cell Sci; A Wntless-SEC12 complex on the ER membrane regulates early Wnt secretory vesicle assembly and mature ligand export.; Authors - Jiaxin Sun ; KD Validated
rat	WB	Ann Transl Med; Proteomics and metabolomics of raw rhubarb and wine-processed rhubarb in the treatment of rats with intracerebral hemorrhage.; Authors - Kun Wang
human	WB	Epigenomics; Methylation of TMEM176A, a key ERK signaling regulator, is a novel synthetic lethality marker of ATM inhibitors in human lung cancer.; Authors - Hongxia Li