Anticorps Polyclonal de lapin anti-SAP18

SAP18 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

Avis (1)

N° de cat : 13841-1-AP

Synonymes

Sin3-associated polypeptide p18, SAP18P, GIG38, Cell growth-inhibiting gene 38 protein, 2HOR0202

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Galerie de données de validation

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  WB analysis of K-562 using 13841-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13841-1-AP (SAP18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 13841-1-AP

  IP result of anti-SAP18 (IP:13841-1-AP, 4ug; Detection:13841-1-AP 1:800) with K-562 cells lysate 1600 ug.

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  IHC staining of human cervical cancer using 13841-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13841-1-AP (SAP18 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 13841-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13841-1-AP (SAP18 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 13841-1-AP

  Immunofluorescent analysis of Hela cells, using SAP18 antibody 13841-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules K-562
• Résultats positifs en IP: cellules K-562,
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

13841-1-AP cible SAP18 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, singe
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SAP18 Protéine recombinante Ag4795
• Nom complet: Sin3A-associated protein, 18kDa
• Masse moléculaire calculée: 153 aa, 18 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC030836
• Symbole du gène: SAP18
• Identification du gène (NCBI): 10284
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SAP18 is part of the SIN3-repressing complex, which enhances the ability of SIN3-HDAC1-mediated transcriptional repression. When tethered to the promoter, it can direct the formation of a repressive complex to core histone proteins. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism

Publications

Species	Application	Title
human	WB	iScience; Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.; Authors - Mio Iwasaki
monkey	WB,IP,IF	Vet Microbiol; Nsp10-interacting host protein SAP18 restricts PEDV replication in Marc-145 cells via enhancing dephosphorylation of RIG-I; Authors - Naling Yang ; KD Validated
human	WB	iScience; RNPS1 in PSAP complex controls periodic pre-mRNA splicing over the cell cycle; Authors - Kazuhiro Fukumura

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sophie (Verified Customer) (09-15-2025)

N/A

• Applications: Western Blot, Immunoprecipitation

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