Anticorps Polyclonal de lapin anti-SAMD9L

• Phare
• Validé par KD/KO

SAMD9L Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 25173-1-AP

Synonymes

C7orf6, DRIF2, KIAA2005, SAMD9L, UEF, UEF1

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Galerie de données de validation

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  WB analysis using 25173-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 25173-1-AP (SAMD9L antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 25173-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 25173-1-AP (SAMD9L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 25173-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25173-1-AP (SAMD9L Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 25173-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25173-1-AP (SAMD9L Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse spleen using 25173-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 25173-1-AP (SAMD9L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 25173-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25173-1-AP (SAMD9L Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 25173-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25173-1-AP (SAMD9L Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 25173-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25173-1-AP (SAMD9L Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of 3T3-L1 using 25173-1-AP

  Immunofluorescent analysis of (4% PFA) fixed 3T3-L1 cells using SAMD9L antibody (25173-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of COLO 320 using 25173-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed COLO 320 cells using 25173-1-AP (SAMD9L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu rénal de rat, cellules COLO 320
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu cérébral humain, tissu de cancer du côlon humain, tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules COLO 320, cellules 3T3-L1

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

25173-1-AP cible SAMD9L dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SAMD9L Protéine recombinante Ag18541
• Nom complet: sterile alpha motif domain containing 9-like
• Masse moléculaire calculée: 1584 aa, 185 kDa
• Poids moléculaire observé: 160-180 kDa
• Numéro d’acquisition GenBank: BC127118
• Symbole du gène: SAMD9L
• Identification du gène (NCBI): 219285
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sterile alpha motif domain-containing protein 9-like(SAMD9L), also named as C7orf6, DRIF2, KIAA2005 and UEF, is a cytoplasmic protein that acts as a tumor suppressor but also plays a key role in cell proliferation and the innate immune response to viral infection. SAMD9L protein is an endosome fusion facilitator which causes myeloid malignancies in mice similar to human diseases with monosomy 7. The SAMD9L protein also appears to play an important role in the brain, particularly the part of the brain that coordinates movement (the cerebellum). SAMD9L has two isoforms with MW 185 kDa and 47 kDa.

Publications

Species	Application	Title
human	WB	Nat Med; Clinical evolution, genetic landscape and trajectories of clonal hematopoiesis in SAMD9/SAMD9L syndromes.; Authors - Sushree S Sahoo
human	WB	PLoS Pathog; A paralogous pair of mammalian host restriction factors form a critical host barrier against poxvirus infection.; Authors - Xiangzhi Meng ; KO Validated
human	IHC	Immunobiology; Shared biomarkers of multi-tissue origin for primary Sjogren's syndrome and their importance in immune microenvironment alterations; Authors - Tiantian Cai
human	WB,IF	Brain Commun; New spinocerebellar ataxia subtype caused by SAMD9L mutation triggering mitochondrial dysregulation (SCA49).; Authors - Marc Corral-Juan
human	WB	Exp Cell Res; Viral host range factors antagonize pathogenic SAMD9 and SAMD9L variants; Authors - Stine Gahr
human	IHC	J Inflamm Res; Integrated Analysis Identifies Upregulated SAMD9L as a Potential Biomarker Correlating with the Severity of Primary Sjögren's Syndrome; Authors - Donghai Zhou