Anticorps Polyclonal de lapin anti-SALL4

• Phare
• Validé par KD/KO

SALL4 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 24500-1-AP

Synonymes

DRRS, HSAL4, sal like 4 (Drosophila), Sal like protein 4, SALL4, Zinc finger protein SALL4, ZNF797

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Galerie de données de validation

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  WB analysis of HepG2 using 24500-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24500-1-AP (SALL4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 24500-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24500-1-AP (SALL4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 24500-1-AP

  IP result of anti-SALL4 (IP:24500-1-AP, 4ug; Detection:24500-1-AP 1:600) with HepG2 cells lysate 3600ug.

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  IHC staining of human testis using 24500-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 24500-1-AP (SALL4 Antibody) at dilution of 1:50.

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  IHC staining of human testis using 24500-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 24500-1-AP (SALL4 Antibody) at dilution of 1:50.

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  IHC staining of human ovary tumor using 24500-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 24500-1-AP (SALL4 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human ovary tumor using 24500-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 24500-1-AP (SALL4 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of Caco-2 using 24500-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using SALL4 antibody (24500-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HepG2,
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu testiculaire humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules Caco-2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

24500-1-AP cible SALL4 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SALL4 Protéine recombinante Ag17480
• Nom complet: sal-like 4 (Drosophila)
• Masse moléculaire calculée: 1053 aa, 112 kDa
• Poids moléculaire observé: 66-75 kDa
• Numéro d’acquisition GenBank: BC111714
• Symbole du gène: SALL4
• Identification du gène (NCBI): 57167
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sal-like protein 4 (SALL4), also named Zinc finger protein 797, contains 7 C2H2-type zinc fingers and belongs to the sal C2H2-type zinc-finger protein family. SALL4 is a transcription factor that exists in two splice isoforms, SALL4a and SALL4b, and regulates transcription in embryonic stem cells, hematopoiesis, and acute myeloid leukemia. SALL4 is activated in fetal liver tissue, silenced in adult liver tissue, and reexpressed as an oncofetal protein in hepatocellular carcinoma (PMID: 26892498, 23758232). SALL4 has two isoforms with predicted molecular weights of 112 kDa (SALL4A) and 66 kDa (unmodified SALL4B), and SALL4B was found to be the dominant endogenous isoform in HCC cell lines. Ectopically expressed SALL4B migrated at 95 kDa modified by ubiquitination, 130 kDa modified by sumoylation (PMID: 23012367).

Publications

Species	Application	Title
human	IF	Sci Rep; Hepatocellular Carcinoma-propagating Cells are Detectable by Side Population Analysis and Possess an Expression Profile Reflective of a Primitive Origin.; Authors - Honghai Xia
human	IHC,WB	Cell Signal; miR-296-5p suppresses stem cell potency of hepatocellular carcinoma cells via regulating Brg1/Sall4 axis.; Authors - Dong-Min Shi
human	WB	Onco Targets Ther; PMID: 31632072; Authors - Chiung-Chi Cheng
human	WB	Sci Rep; MicroRNA-33b Inhibits Breast Cancer Metastasis by Targeting HMGA2, SALL4 and Twist1.; Authors - Yancheng Lin
human	WB	Pathol Int; Salt-like transcription factor 4 promotes laryngeal cancer progression through transcriptional activation of ubiquitin-specific protease 21 to stabilize Yin Yang 1; Authors - Qing-Dong Wang
human	WB,IHC,IF	J Mol Histol; Expression and clinical significance of SALL4 and β-catenin in colorectal cancer.; Authors - Liliang Hao