Anticorps Polyclonal de lapin anti-S100A5

S100A5 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 17924-1-AP

Synonymes

Protein S 100D, Protein S100 A5, S100A5, S100D

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Galerie de données de validation

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  WB analysis of HepG2 using 17924-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17924-1-AP (S100A5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 17924-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 17924-1-AP (S100A5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 17924-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17924-1-AP (S100A5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 17924-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 17924-1-AP (S100A5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human meningioma using 17924-1-AP

  Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 17924-1-AP (S100A5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human meningioma using 17924-1-AP

  Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 17924-1-AP (S100A5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human meningioma using 17924-1-AP

  Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 17924-1-AP (S100A5 Antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human meningioma using 17924-1-AP

  Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 17924-1-AP (S100A5 Antibody) at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human brain using 17924-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17924-1-AP (S100A5 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 17924-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17924-1-AP (S100A5 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 17924-1-AP

  Immunofluorescent analysis of HepG2 cells using 17924-1-AP (S100A5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules SH-SY5Y, tissu hépatique humain, tissu rénal humain
• Résultats positifs en IHC: tissu de méningiome humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

17924-1-AP cible S100A5 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: S100A5 Protéine recombinante Ag12342
• Nom complet: S100 calcium binding protein A5
• Masse moléculaire calculée: 110 aa, 13 kDa
• Poids moléculaire observé: 11 kDa
• Numéro d’acquisition GenBank: BC093955
• Symbole du gène: S100A5
• Identification du gène (NCBI): 6276
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IHC,IF	Adv Sci (Weinh); S100A5 Attenuates Efficiency of Anti-PD-L1/PD-1 Immunotherapy by Inhibiting CD8+ T Cell-Mediated Anti-Cancer Immunity in Bladder Carcinoma; Authors - Huihuang Li
mouse	IHC	Mol Cell Proteomics; The Membrane Proteome of Sensory Cilia to the Depth of Olfactory Receptors.; Authors - Katja Kuhlmann