Anticorps Polyclonal de lapin anti-S100A4

• Phare
• Validé par KD/KO

S100A4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

54

Avis (1)

N° de cat : 16105-1-AP

Synonymes

FSP1, Fibroblast-specific protein-1, Fibroblast-specific protein 1, Fibroblast Specific Protein, CAPL

---

Galerie de données de validation

• 

  WB analysis using 16105-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16105-1-AP (S100A4 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16105-1-AP

  WB result of S100A4 antibody (16105-1-AP; 1:200000; incubated at room temperature for 1.5 hours) with sh-Control and sh-S100A4 transfected HeLa cells.

• 

  IHC staining of mouse spleen using 16105-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 16105-1-AP (S100A4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16105-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16105-1-AP (S100A4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 16105-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16105-1-AP (S100A4 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human breast cancer using 16105-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16105-1-AP (S100A4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 16105-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16105-1-AP (S100A4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 16105-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 16105-1-AP (S100A4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 16105-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 16105-1-AP (S100A4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 16105-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16105-1-AP (S100A4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 16105-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16105-1-AP (S100A4 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 16105-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using S100A4 antibody (16105-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 16105-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human S100A4 (16105-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du côlon humain, tissu de cancer du pancréas humain, tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 26 publications below
• IHC: See 15 publications below
• IF: See 27 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below

Informations sur le produit

16105-1-AP cible S100A4 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: S100A4 Protéine recombinante Ag9019
• Nom complet: S100 calcium binding protein A4
• Masse moléculaire calculée: 101 aa, 12 kDa
• Poids moléculaire observé: 10-12 kDa
• Numéro d’acquisition GenBank: BC016300
• Symbole du gène: S100A4
• Identification du gène (NCBI): 6275
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

S100A4 is a member of the S100 family of calcium-binding proteins. The S100 family members have been involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A4 is known to localize to and function in the nucleus, cytoplasm of cells, and the extracellular space. S100A4 has also been shown to be associated with tumor growth, motility, invasion, metastasis, angiogenesis, apoptosis, and chemoresistance. It is a fibroblast-specific protein associated with mesenchymal cell morphology and motility, is expressed during epithelial-mesenchymal transformations (EMT) in vivo (PMID: 9362334). It is a specific prognostic marker for renal survival in patients with IgAN (PMID: 16105038). It is also an improved marker for lung fibroblasts that could be useful for investigating the pathogenesis of pulmonary fibrosis(PMID: 15618458). Overexpression of S100A4 is correlated with a worse prognosis inpatients with various types of cancer.

Publications

Species	Application	Title
human	WB	Cell; Suppression of Enhancer Overactivation by a RACK7-Histone Demethylase Complex.; Authors - Hongjie Shen ; KO Validated
human	IHC	J Hematol Oncol; Proteogenomic insights into the biology and treatment of pancreatic ductal adenocarcinoma; Authors - Yexin Tong
human	WB	Nat Commun; Co-evolution of matrisome and adaptive adhesion dynamics drives ovarian cancer chemoresistance.; Authors - Elina A Pietilä
mouse	WB	ACS Appl Mater Interfaces; Smart Manganese Dioxide-Based Lanthanide Nanoprobes for Triple-Negative Breast Cancer Precise Gene Synergistic Chemodynamic Therapy.; Authors - Liyan Ming
human	WB,IF	Oncogene; Stromal nicotinamide N-methyltransferase orchestrates the crosstalk between fibroblasts and tumour cells in oral squamous cell carcinoma: evidence from patient-derived assembled organoids; Authors - Hui Zhao
human	IF	J Crohns Colitis; Exosomal miR-103a-3p from Crohn's creeping fat-derived ASCs contributes to intestinal fibrosis by targeting TGFBR3 and activating fibroblasts; Authors - Wenwei Qian

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Marco (Verified Customer) (07-09-2024)

Does exactly what it should do in cardiac rat cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: NRVCMs

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C

P53 Polyclonal antibody

10442-1-AP

S100 Beta Polyclonal antibody

15146-1-AP

MYH9 Polyclonal antibody

11128-1-AP

S100A6 Polyclonal antibody

10245-1-AP

S100A6 Monoclonal antibody

66098-1-Ig

E-cadherin Polyclonal antibody

20874-1-AP

E-cadherin Monoclonal antibody

60335-1-Ig

S100 Beta Polyclonal antibody

15146-1-AP

MMP2 Polyclonal antibody

10373-2-AP

NOV Polyclonal antibody

55449-1-AP