Anticorps Polyclonal de lapin anti-S100A12

S100A12 Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 16630-1-AP

Synonymes

CAAF1, CAGC, Calgranulin C, CGRP, EN RAGE, ENRAGE, MRP6, Neutrophil S100 protein, p6, Protein S100 A12, S100A12

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Galerie de données de validation

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  IHC staining of human tonsillitis using 16630-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16630-1-AP (S100A12 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human tonsillitis using 16630-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16630-1-AP (S100A12 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human tonsillitis using 16630-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16630-1-AP (S100A12 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 16630-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16630-1-AP (S100A12 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 16630-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16630-1-AP (S100A12 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 16630-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16630-1-AP (S100A12 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 3 publications below

Informations sur le produit

16630-1-AP cible S100A12 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: S100A12 Protéine recombinante Ag9961
• Nom complet: S100 calcium binding protein A12
• Masse moléculaire calculée: 92 aa, 11 kDa
• Numéro d’acquisition GenBank: BC070294
• Symbole du gène: S100A12
• Identification du gène (NCBI): 6283
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

S100A12 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. S100A12 is expressed abundantly in granulocytes, and has been implicated to play an important role on inflammatory reactions in various disease states.

Publications

Species	Application	Title
	IHC	Biol Reprod; Effects of progesterone and interferon tau on ovine endometrial phosphate, calcium, and vitamin D signaling.; Authors - Claire Stenhouse
human	WB,IHC	Connect Tissue Res; S100A12 is involved in the pathology of osteoarthritis by promoting M1 macrophage polarization via the NF-κB pathway; Authors - Yi Zhang
human	IHC	Redox Biol; Lipoxin A4 ameliorates knee osteoarthritis progression in rats by antagonizing ferroptosis through activation of the ESR2/LPAR3/Nrf2 axis in synovial fibroblast-like synoviocytes; Authors - Zhehan Hu