Anticorps Polyclonal de lapin anti-RXRG

RXRG Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, PLA, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 11129-1-AP

Synonymes

NR2B3, Retinoid X receptor gamma, retinoid X receptor, gamma, RXRC, RXRG

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Galerie de données de validation

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  WB analysis of mouse heart using 11129-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11129-1-AP (RXRG Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis of mouse ovary using 11129-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 11129-1-AP (RXRG Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11129-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11129-1-AP (RXRG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of rat eye using 11129-1-AP

  Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 11129-1-AP (RXRG antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat eye using 11129-1-AP

  Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 11129-1-AP (RXRG antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 11129-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11129-1-AP (RXRG antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 11129-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11129-1-AP (RXRG antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules Jurkat, tissu ovarien de souris
• Résultats positifs en IHC: tissu oculaire de rat, tissu de muscle squelettique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

11129-1-AP cible RXRG dans les applications de WB, IF, PLA, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RXRG Protéine recombinante Ag1611
• Nom complet: retinoid X receptor, gamma
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 51-55 kDa
• Numéro d’acquisition GenBank: BC012063
• Symbole du gène: RXRG
• Identification du gène (NCBI): 6258
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RXRG, also named as, Retinoic acid receptor RXR-gamma, is a 463 amino acid protein, which localize in the nucleus and may form homodimer. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5.

Publications

Species	Application	Title
mouse	PLA	Aging (Albany NY); Cholecalciferol (vitamin D3) has a direct protective activity against interleukin 6-induced atrophy in C2C12 myotubes.; Authors - Maraiza Alves Teixeira
rat	IF	Am J Physiol Lung Cell Mol Physiol; Inhaled vitamin A is more effective than intramuscular dosing in mitigating hyperoxia-induced lung injury in a neonatal rat model of bronchopulmonary dysplasia.; Authors - Craig A Gelfand
human	WB	Cells; EGFR/ErbB Inhibition Promotes OPC Maturation up to Axon Engagement by Co-Regulating PIP2 and MBP.; Authors - Emanuela Nocita