Anticorps Polyclonal de lapin anti-RUNX1T1

• Phare
• Validé par KD/KO

RUNX1T1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 15494-1-AP

Synonymes

AML1T1, CBFA2T1, CDR, Cyclin D related protein, Eight twenty one protein, ETO, MTG8, MTG8b, Protein CBFA2T1, Protein ETO, Protein MTG8, RUNX1T1, ZMYND2

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Galerie de données de validation

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  WB analysis of HEK-293 using 15494-1-AP

  WB result of RUNX1T1 antibody (15494-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RUNX1T1 transfected HEK-293 cells.

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  WB analysis using 15494-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15494-1-AP (RUNX1T1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15494-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15494-1-AP (RUNX1T1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 15494-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15494-1-AP (RUNX1T1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 15494-1-AP

  IP result of anti-RUNX1T1 (IP:15494-1-AP, 4ug; Detection:15494-1-AP 1:800) with mouse brain tissue lysate 3600ug.

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  IHC staining of mouse brain using 15494-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15494-1-AP (RUNX1T1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 15494-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15494-1-AP (RUNX1T1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HEK-293, cellules HepG2, cellules Jurkat, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

15494-1-AP cible RUNX1T1 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RUNX1T1 Protéine recombinante Ag7892
• Nom complet: runt-related transcription factor 1; translocated to, 1 (cyclin D-related)
• Masse moléculaire calculée: 68 kDa
• Poids moléculaire observé: 64-69 kDa
• Numéro d’acquisition GenBank: BC005850
• Symbole du gène: RUNX1T1
• Identification du gène (NCBI): 862
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RUNX1T1 is a putative zinc finger transcription factor and oncoprotein. In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 gene fused to the 3'-region of this gene. Various transcript of the fusion gene has been reported. RUNX1T1 exists some isoforms with MV 68, 67,64, 48 and 44 kDa. The calcualted molecular weight of RUNX1T1 is 67 kDa, but modified RUNX1T1is about 70-75 kDa.

Publications

Species	Application	Title
human	WB	Cell Res; FTO-dependent demethylation of N6-methyladenosine regulates mRNA splicing and is required for adipogenesis.; Authors - Xu Zhao
sheep	WB,IF	Int J Mol Sci; Characterization of RUNX1T1, an Adipogenesis Regulator in Ovine Preadipocyte Differentiation.; Authors - Kaiping Deng ; KD Validated
human	WB	J Biol Chem; Lactate promotes neuronal differentiation of SH-SY5Y cells by lactate-responsive gene sets through NDRG3-dependent and -independent manners; Authors - Yidan Xu