Anticorps Polyclonal de lapin anti-RUNX1 (middle)

• Phare
• Validé par KD/KO

RUNX1 (middle) Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

20

Avis (2)

N° de cat : 25315-1-AP

Synonymes

RUNX1, CBF-alpha-2, CBFA2, CBF alpha 2, AMLCR1

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Galerie de données de validation

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  WB analysis using 25315-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25315-1-AP (RUNX1 (middle) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 25315-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25315-1-AP (RUNX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 25315-1-AP

  IP result of anti-RUNX1 (middle) (IP:25315-1-AP, 4ug; Detection:25315-1-AP 1:300) with Jurkat cells lysate 3440ug.

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  IHC staining of human colon cancer using 25315-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25315-1-AP (RUNX1 (middle) antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 25315-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25315-1-AP (RUNX1 (middle) antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 25315-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25315-1-AP (RUNX1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 25315-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25315-1-AP (RUNX1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 25315-1-AP

  Immunofluorescent analysis of HepG2 cells using 25315-1-AP (RUNX1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of Jurkat using 25315-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.5 ug Anti-Human RUNX1 (middle) (25315-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, tissu de thymus de souris
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2
• Résultats positifs en FC (Intra): cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 17 publications below
• IHC: See 5 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

25315-1-AP cible RUNX1 (middle) dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RUNX1 (middle) Protéine recombinante Ag17838
• Nom complet: runt-related transcription factor 1
• Masse moléculaire calculée: 480 aa, 52 kDa
• Poids moléculaire observé: 48-55 kDa
• Numéro d’acquisition GenBank: BC136381
• Symbole du gène: RUNX1
• Identification du gène (NCBI): 861
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Runt-related transcription factor 1 (RUNX1), also named AML1 or CBF alpha 2, is a 453 amino acid protein, which contains one Runt domain. RUNX1 localizes in the nucleus and is expressed in all tissues except the brain and heart. RUNX1 is involved in hematopoiesis and is frequently targeted in human leukemia by chromosomal translocations that fuse the DNA-binding domain of RUNX1 to other transcription factors and corepressor molecules. In addition to its role in leukemogenesis, RUNX1 is also involved in sensory neuron diversification. RUNX1 exists in some isoforms with a range of MV 20-52 kDa. The calculated molecular weight of isoform 1 is 49 kDa, but the modified protein is about 49-55 kDa.

Publications

Species	Application	Title
mouse	WB	Immunity; The Transcription Factor Runx3 Establishes Chromatin Accessibility of cis-Regulatory Landscapes that Drive Memory Cytotoxic T Lymphocyte Formation.; Authors - Dapeng Wang
mouse	IHC, WB	Genes Dev; ASCL1 represses a SOX9+ neural crest stem-like state in small cell lung cancer.; Authors - Rachelle R Olsen
human	WB	J Hematol Oncol; RUNX1 mutations promote leukemogenesis of myeloid malignancies in ASXL1-mutated leukemia.; Authors - Rabindranath Bera
rat	WB	Int Immunopharmacol; miR-9 targeting RUNX1 improves LPS-induced alveolar hypercoagulation and fibrinolysis inhibition through NF-κB inactivation in ARDS; Authors - Chuan Xiao
human	WB	J Cell Physiol; Inhibition of RUNX1 blocks the differentiation of lung fibroblasts to myofibroblasts.; Authors - Shubham Dubey ; KD Validated
human	WB	Eur J Pharmacol; Vitamin D3 analogue calcipotriol inhibits the profibrotic effects of transforming growth factor- β1 on pancreatic stellate cells; Authors - Meifang Zheng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

SD (Verified Customer) (07-08-2022)

Excellent Antibody!

• Applications: Western Blot
• Cell Tissue Type: H9C2

RUNX1 (middle) Antibody Western Blot validation ( dilution) in H9C2 (Cat no:25315-1-AP)

FH

P (Verified Customer) (12-01-2021)

Excellent

• Applications: Western Blot
• Cell Tissue Type: fibroblast

RUNX1 (middle) Antibody Western Blot validation ( dilution) in fibroblast (Cat no:25315-1-AP)