Anticorps Polyclonal de lapin anti-RUNX1 (N-terminal)

RUNX1 (N-terminal) Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 19555-1-AP

Synonymes

AML1, AML1 EVI 1, AMLCR1, CBF alpha 2, CBFA2, EVI 1, Oncogene AML 1, PEA2 alpha B, PEBP2 alpha B, PEBP2aB, RUNX1, RUNX1 (N-terminal)

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Galerie de données de validation

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  WB analysis of mouse liver using 19555-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Sp2/0 using 19555-1-AP

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 19555-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 19555-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 19555-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 19555-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 19555-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 19555-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 19555-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Sp2/0 using 19555-1-AP

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Sp2/0 using 19555-1-AP

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 19555-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 19555-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 19555-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 19555-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 19555-1-AP (RUNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 19555-1-AP

  IP result of anti-RUNX1 (N-terminal) (IP:19555-1-AP, 4ug; Detection:19555-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules HEK-293, cellules HL-60, cellules Jurkat, cellules MCF-7, cellules Raji, cellules SH-SY5Y, cellules Sp2/0, tissu de thymus de souris, tissu splénique de souris
• Résultats positifs en IP: tissu hépatique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

19555-1-AP cible RUNX1 (N-terminal) dans les applications de WB, IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: runt-related transcription factor 1
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 48-55 kDa
• Numéro d’acquisition GenBank: NM_001754
• Symbole du gène: RUNX1
• Identification du gène (NCBI): 861
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Runt-related transcription factor 1 (RUNX1), also named AML1 or CBF alpha 2, is a 453 amino acid protein, which contains one Runt domain. RUNX1 localizes in the nucleus and is expressed in all tissues except the brain and heart. RUNX1 is involved in hematopoiesis and is frequently targeted in human leukemia by chromosomal translocations that fuse the DNA-binding domain of RUNX1 to other transcription factors and corepressor molecules. In addition to its role in leukemogenesis, RUNX1 is also involved in sensory neuron diversification. RUNX1 exists in some isoforms with a range of MV 20-52 kDa. The calculated molecular weight of isoform 1 is 49 kDa, but the modified protein is about 49-55 kDa. This antibody (19555-1-AP) can't recognize 37 kDa isoform 11.

Publications

Species	Application	Title
mouse	WB	Protein Expr Purif; Msx1 cooperates with Runx1 for inhibiting myoblast differentiation.; Authors - Guoqiang Zhou
human	WB,CoIP	Mol Cells; RUNX1 Ameliorates Rheumatoid Arthritis Progression through Epigenetic Inhibition of LRRC15; Authors - Hao Ding
rat	ChIP,WB	Respir Res; RUNX1 targeting AKT3 promotes alveolar hypercoagulation and fibrinolytic inhibition in LPS induced ARDS; Authors - Chuan Xiao