Anticorps Polyclonal de lapin anti-RTN4/NOGO

• Phare
• Validé par KD/KO

RTN4/NOGO Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 10950-1-AP

Synonymes

NOGO, RTN4, Nbla10545, Nbla00271, My043

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Galerie de données de validation

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  WB analysis using 10950-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10950-1-AP (RTN4/NOGO antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10950-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10950-1-AP (RTN4/NOGO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 10950-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10950-1-AP (RTN4/NOGO antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10950-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10950-1-AP (RTN4/NOGO antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 10950-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10950-1-AP (RTN4/NOGO antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 10950-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10950-1-AP (RTN4/NOGO antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of SH-SY5Y using 10950-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using RTN4/NOGO antibody (10950-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A375 using 10950-1-AP

  Immunofluorescent analysis of A375 cells using 10950-1-AP (RTN4/NOGO antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 10950-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human RTN4/NOGO (10950-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A375, cellules A549, cellules HepG2, cellules SH-SY5Y
• Résultats positifs en IHC: tissu cérébral de souris, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules SH-SY5Y, cellules A375
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IF: See 3 publications below

Informations sur le produit

10950-1-AP cible RTN4/NOGO dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RTN4/NOGO Protéine recombinante Ag1392
• Nom complet: reticulon 4
• Masse moléculaire calculée: 130 kDa
• Poids moléculaire observé: 45-50 kDa
• Numéro d’acquisition GenBank: BC012619
• Symbole du gène: NOGO
• Identification du gène (NCBI): 57142
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER) (PMID: 18177508). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). They are involved in shaping the tubular endoplasmic reticulum network, membrane trafficking, inhibition of axonal growth, and apoptosis (PMID: 24218324). Four mammalian reticulons (RTN1-4) exist. RTN4 (also known as Neurite outgrowth inhibitor or Nogo) is a myelin-associated neurite growth inhibitory protein. Some isoforms of RTN4 have been described. RTN4A (Nogo-A, runs at ~200 kDa), RTN4B (Nogo-B, 40-55 kDa), and RTN4C (Nogo-C, 22-25 kDa) are three major isoforms (PMID: 31092426; 16469703).

Publications

Species	Application	Title
human	WB	PLoS Biol; Reticulon and CLIMP-63 regulate nanodomain organization of peripheral ER tubules.; Authors - Guang Gao ; KD Validated
human	WB,IF	Autophagy; Dengue and Zika viruses subvert reticulophagy by NS2B3-mediated cleavage of FAM134B.; Authors - Nicholas J Lennemann
human	WB	J Cell Sci; SEC24A facilitates colocalization and calcium flux between endoplasmic reticulum and mitochondria.; Authors - Tamutenda Chidawanyika
rat	WB	Drug Des Devel Ther; Tanshinone IIA Promotes Axonal Regeneration in Rats with Focal Cerebral Ischemia Through the Inhibition of Nogo-A/NgR1/RhoA/ROCKII/MLC Signaling.; Authors - Jing Wang
mouse	WB	Neurol Res; Erythropoietin attenuates axonal injury after middle cerebral artery occlusion in mice.; Authors - Rongliang Wang
mouse	WB	NPJ Parkinsons Dis; Deficiency of Perry syndrome-associated p150Glued in midbrain dopaminergic neurons leads to progressive neurodegeneration and endoplasmic reticulum abnormalities; Authors - Jia Yu